| 货号 | AF1995-SP |
| 别名 | C9orf2; H7365member 1; Tomoregulin-1; TR-1; Transmembrane protein with EGF-like and one follistatin-like domain; transmembrane protein with EGF-like and two follistatin-like domains 1 | 全称 | Transmembrane Protein with EFG-like and two Follistatin-like Domains |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse TMEFF1/Tomoregulin‑1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross‑reactivity with recombinant human (rh) TMEFF1 is observed and less than 5% cross-reactivity with rhTMEFF2 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8577 (Human); 230157 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse TMEFF1/Tomoregulin‑1 Ser37-Thr320 Accession # Q6PFE7 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | TMEFF1, also known as tomoregulin 1 is a type I transmembrane glycoprotein that contains two follistatin modules and an EGF domain in its extracellular domain, a transmembrane domain and a short cytoplasmic tail. The extracellular domain can be released as a soluble protein. TMEFF1 selectively regulates nodal but not activin signaling through direct binding to the nodal co-receptor, Cripto. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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TMEFF1/Tomoregulin‑1 in Mouse Spinal Cord. TMEFF1/Tomoregulin‑1 was detected in perfusion fixed frozen sections of mouse spinal cord using 15 µg/mL Goat Anti-Mouse TMEFF1/Tomoregulin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1995) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
