| 货号 | AF1899-SP |
| 别名 | FGF homologous factor receptor; FGF receptor-like protein 1; FGFR5; FGFR-5; FGFRL1; FGFR-like protein; FHFR; Fibroblast growth factor receptor 5; fibroblast growth factor receptor-like 1 | 全称 | Fibroblast Growth Factor Receptor-like 1 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse FGF R5 beta in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) FGF R1 alpha (IIIb), recombinant mouse (rm) FGF R2 beta (IIIc), rmFGF R3 alpha (IIIb), rhFGF R3 alpha (IIIc), rhFGF R1 beta (IIIc), rmFGF R2 beta (IIIb), rhFGF R1 alpha (IIIc), rmFGF R2 alpha (IIIc), rhFGF R1 beta (IIIb), rmFGF R2 alpha (IIIb), rmFGF R2, and rmFGF R4 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 53834 (Human); 116701 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The murine Fgfrl1 receptor is essential for the development of the metanephric kidney. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse FGF R5 beta Ala21-Pro374 Accession # Q91V87 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Fibroblast growth factor receptor 5 (FGF R5), also known as FGRL1, is a 65 kDa transmembrane member of the FGF receptor family (1). Mature mouse FGF R5 consists of a 354 amino acid (aa) extracellular domain (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 134 aa cytoplasmic domain (2). FGF R5 is distinct from other FGF receptor family members in that it does not contain a cytoplasmic tyrosine kinase domain (2, 3). Within the ECD, mouse FGF R5 shares 94% and 98% aa sequence identity with human and rat FGF R5, respectively. Alternate splicing results in an isoform that lacks the first Ig-like domain (2). FGF R5 is widely expressed, and expression of two species of mRNA in cartilage and pancreas may indicate the presence of both splice forms (2-6). Both the full length and truncated isoforms of FGF R5 bind FGF basic; the full length form additionally binds to heparin (2, 6). FGF R5 may function as a decoy receptor by binding FGF but not transducing its mitogenic signals (6). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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FGF R5/FGFRL1 in Mouse Lung. FGF R5/FGFRL1 was detected in perfusion fixed frozen sections of mouse lung using Goat Anti-Mouse FGF R5/FGFRL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1899) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane and cytoplasm of alveolar cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
