| 货号 | AF1888-SP |
| 别名 | AMRF; CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 2(lysosomal integral membrane protein II); CD36 antigen; CD36L2; CD36L2LIMP II; HLGP85; LGP85; LIMP-2,85 kDa lysosomal sialoglycoprotein scavenger receptor class B, member 2; LIMPIICD36 antigen-like 2; LPG85; lysosome membrane protein 2; Lysosome membrane protein II; SCARB2; Scavenger receptor class B member 2,85 kDa lysosomal membrane sialoglycoprotein; scavenger receptor class B, member 2; SR-B2; SR-BII | 全称 | Lysosomal Integral Membrane Protein II |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) Intracellular Staining by Flow Cytometry(0.25 µg/106cells) |
| 目标/特异性 | Detects mouse LIMPII/SR‑B2 Lumenal Loop in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross‑reactivity with recombinant human (rh) LIMPII is observed and less than 1% cross‑reactivity with rhCD36 and rhSR‑B1 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Intracellular Staining by Flow Cytometry: 0.25 µg/106cells |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 950 (Human); 12492 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. HSL-knockout mouse testis exhibits class B scavenger receptor upregulation and disrupted lipid raft microdomains. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse LIMPII/SR‑B2 Lumenal Loop Arg27-Thr432 Accession # O35114 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | LIMPII (Lysosomal Integral Membrane Protein II), also known as LPG85 (85 kDa lysosomal membrane sialoglycoprotein) and CD36 antigen-like 2 (CD36L2), is a major lysosomal membrane protein. It belongs to the scavenger receptor class B subfamily and is designated member 2 (SR-B2). Other mammalian members of this family include SR-B1 (alternatively known as Cla-1 and CD36L1), and SR-B3 (CD36) (1‑3). SR-B/CD36 family members are type III integral membrane proteins with an N‑ as well as a C-terminal cytoplasmic tail, and a large extracellular (or luminal in the case of LIMPII) loop containing similarly spaced cysteine residues and multiple glycosylation sites. The C-terminal cytoplasmic tail has a di-leucine-based motif that mediates effective lysosomal targeting. LIMPII is expressed on all tissues and cell types examined so far, including activated platelets. LIMPII binds thrombospondin-1, but the biological significance of this interaction is not known. LIMPII‑thrombospondin interaction may contribute to the pro-adhesive changes of activated platelets during coagulation and inflammation (1). Overexpression of LIMPII causes an enlargement of early and late endosomes, suggesting that LIMPII may play a role in lysosome/endosome biogenesis (4). Mice deficient in LIMPII are impaired in membrane transport processes, resulting in ureteric pelvic junction obstruction, deafness and peripheral neuropathy (5). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of LIMPII/SR-B2 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Goat Anti-Mouse LIMPII/SR-B2 Affinity-purified Polyclonal Antibody (Catalog # AF1888, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. |
