| 货号 | AF1885-SP |
| 别名 | T Cell immunoglobulin and mucin domain protein 2; Tim2 | 全称 | T Cell Immunoglobulin Mucin-2 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(1-15 µg/mL) |
| 目标/特异性 | Detects mouse TIM-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross‑reactivity with recombinant human TIM-1 and recombinant mouse TIM-3 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 1-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 171284 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse TIM‑2 His22-Asn229 Accession # NP_599010 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | TIM-2 is a type I membrane protein and is a member of the TIM protein family. This protein family is characterized by their conserved immunoglobulin and mucin domains, as well as by their expression on activated T cells. TIM-2 is a receptor for Sema 4A, which is expressed on dendritic cells and B cells. Sema 4A has been shown to enhance T cell activation. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
TIM‑2 in Mouse Liver. TIM‑2 was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse TIM‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1885) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sinusoids. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
