| 货号 | AF1866-SP |
| 别名 | antigen identified by monoclonal 1D8; Antigenic surface determinant protein OA3; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 antigen; CD47 glycoprotein; CD47 molecule; CD47; IAP; IAPintegrin associated protein; Integrin-associated protein; leukocyte surface antigen CD47; MER6integrin-associated signal transducer; OA3; Protein MER6; Rh-related antigen |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.25 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse CD47 N-terminal IgV-like in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant human CD47 is observed. |
| 使用方法 | Western Blot: 0.25 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 961 (Human); 16423 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Radiation-induced loss of cell surface CD47 enhances immune-mediated clearance of human papillomavirus-positive cancer. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse CD47 N-terminal IgV-like Gln19-Pro158 Accession # NP_034711 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | CD47, also known as Integrin‑Associated Protein (IAP) and OA3, is a 40‑60 kDa variably glycosylated atypical member of the immunoglobulin superfamily (1, 2). Mouse CD47 is an integral membrane protein that consists of a 122 amino acid (aa) extracellular domain (ECD) with a single Ig‑like domain, five membrane-spanning regions with short intervening loops, and a 16 aa C‑terminal cytoplasmic tail (3). Alternate splicing of mouse CD47 generates an additional isoform with an insertion of 21 aa following the Ig‑like domain (3). Within the N‑terminal ECD, mouse CD47 shares 63% and 84% aa sequence identity with human and rat CD47, respectively. A portion of the N‑terminal ECD can by shed from smooth muscle cells by MMP-2‑mediated proteolysis (4). The ubiquitously expressed CD47 binds to SIRP family members on macrophages, neutrophils, and T cells (5, 6). These interactions prevent macrophage‑mediated clearance of healthy CD47-expressing cells and promote immune cell transmigration across the vascular endothelium (5‑8). CD47 associates in cis with Fas on T cells and enhances Fas‑mediated apoptosis; its ligation promotes T cell anergy and dampens Th1 immune responses (9‑11). CD47 also associates in cis with Integrins alpha 4 beta 1, alpha V beta 3, alpha 2b beta 3, and alpha 2 beta 1 which can positively or negatively modulate Integrin-mediated function (2, 12). In the vasculature, CD47 binding by Thrombospondin‑1 inhibits the angiogenic and vasorelaxant effects of nitric oxide (2, 13, 14). On dendritic cells and myeloma cells, CD47 ligation by TSP‑1 induces giant cell formation and osteoclast differentiation (15). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse CD47 by Western Blot. Western blot shows lysates of mouse ovary tissue and mouse thymus tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse CD47 N-terminal IgV-like Extracellular Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1866) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CD47 at approximately 40-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
