Mouse IL-6 R alpha Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse IL-6 R alpha Affinity Purified Polyclonal Ab (25 UG)

货号： AF1830-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF1830-SP
别名	CD126; IL6R
全称	Interleukin 6 Receptor alpha
反应种属	Mouse
应用	Western Blot,Flow Cytometry,Immunohistochemistry,CyTOF-ready,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects mouse IL‑6 R alpha in ELISAs and Western blots. In sandwich ELISAs, less than 0.3% cross-reactivity with recombinant mouse (rm) IL‑6, recombinant human IL-6 R and rmgp130 is observed.
使用方法	Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Immunohistochemistry: 5-15 µg/mL ELISA Capture (Matched Antibody Pair): 0.2-0.8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize IL‑6-induced proliferation in the T1165.85.2.1 mouse plasmacytoma cell line. Nordan, R. P. and M. Potter (1986) Science 233:566. The Neutralization Dose (ND₅₀) is typically 0.2-1 µg/mL in the presence of 0.25 ng/mL Recombinant Mouse IL‑6.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3570 (Human); 16194 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Species Specificity of ADAM10 and ADAM17 Proteins in Interleukin-6 (IL-6) Trans-signaling and Novel Role of ADAM10 in Inducible IL-6 Receptor Shedding. Authors: Garbers C, Janner N, Chalaris A, Moss ML, Floss DM, Meyer D, Koch-Nolte F, Rose-John S, Scheller J J. Biol. Chem., 2011;286(17):14804-11. Species: Mouse Sample Type: Cell Culture Supernates Application: WB Inactivated Sendai virus particles eradicate tumors by inducing immune responses through blocking regulatory T cells. Authors: Kurooka M, Kaneda Y Cancer Res., 2007;67(1):227-36. Species: Mouse Sample Type: Application: In vivo Transgenic blockade of interleukin 6 transsignaling abrogates inflammation. Authors: Rabe B, Chalaris A, May U, Waetzig GH, Seegert D, Williams AS, Jones SA, Rose-John S, Scheller J Blood, 2007;111(3):1021-8. Species: Mouse Sample Type: Air Pouch Exudate Application: ELISA Development Apoptosis is a natural stimulus of IL6R shedding and contributes to the proinflammatory trans-signaling function of neutrophils. Authors: Chalaris A, Rabe B, Paliga K, Lange H, Laskay T, Fielding CA, Jones SA, Rose-John S, Scheller J Blood, 2007;110(6):1748-55. Species: Mouse Sample Type: Lavage Fluid Application: ELISA Development IL6/sIL6R complex contributes to emergency granulopoietic responses in G-CSF- and GM-CSF-deficient mice. Authors: Walker F, Zhang HH, Matthews V, Weinstock J, Nice EC, Ernst M, Rose-John S, Burgess AW Blood, 2007;111(8):3978-85. Species: Mouse Sample Type: Cell Culture Supernates Application: WB Sequential ELISA to profile multiple cytokines from small volumes. Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG J. Immunol. Methods, 2005;302(1):172-81. Species: Mouse Sample Type: Plasma Application: ELISA Development
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse IL-6 R alpha Leu20-Glu357 Accession # P22272
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Interleukin 6 (IL-6) is a multifunctional cytokine that exerts its activities by binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa ligand binding subunit (IL-6 R alpha /CD126) and a 130 kDa nonligand-binding signal-transducing subunit (gp130/CD130) (1‑4). The mouse IL-6 R alpha cDNA encodes a precursor type I transmembrane protein of 460 amino acids (aa) that contains a 19 aa signal sequence, a 345 aa extracellular ligand binding domain, a 21 aa transmembrane region, and a 75 aa cytoplasmic segment (2). The extracellular segment contains an Ig-like and a fibronectin-type III domain, plus a membrane proximal WSXWS motif. In their extracellular regions, mouse IL-6 R alpha shares 89%, 51% and 50% aa identity with rat, human and porcine IL-6 R alpha, respectively. Unlike gp130 that is expressed ubiquitously, the cellular distribution of IL-6 R alpha is predominantly limited to hepatocytes and leukocyte subpopulations such as monocytes, neutrophils, T and B cells. Soluble IL-6 R alpha has been found in various body fluids (5). Two soluble receptor isoforms that arise either from proteolytic cleavage of the membrane-bound IL-6 R alpha, or by alternative mRNA splicing (reported only in human) have been described (6, 7). Soluble IL-6 R alpha binds IL-6 with an affinity similar to that of the membrane-bound IL-6 R alpha. More importantly, the soluble IL-6 R alpha /IL-6 complex is capable of interacting with the membrane-bound gp130 to activate cells that lack an integral membrane IL-6 R alpha. It has been documented that elevated soluble IL-6 R is associated with numerous diseases including arthritic lesions, multiple myeloma and Crohn’s disease (6, 7).
运输条件	Blue Ice
存放说明	4℃
参考文献	Yamasaki, K. et al. (1988) Science 241:825. Sugita, T. et al. (1990) J. Exp. Med. 171:2001. Hibi, M. et al. (1990) Cell 63:1149. Saito, M. et al. (1992) J. Immunol. 148:4066. Novick, D. et al. (1989) J. Exp. Med. 170:1409. Jones, S.A. et al. (2001) FASEB J. 15:43. Jones, S.A. and S. Rose-John (2002) Biochim. Biophys. Acta 1592:251.

参考图片

IL‑6 R alpha in Mouse Thymus. IL‑6 R alpha was detected in perfusion fixed frozen sections of mouse thymus using Mouse IL‑6 R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1830) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

IL‑6 R alpha in Mouse Thymus. IL‑6 R alpha was detected in perfusion fixed frozen sections of mouse thymus using Mouse IL‑6 R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1830) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Cell Proliferation Induced by IL‑6 and Neutralization by Mouse IL‑6 R alpha Antibody. Recombinant Mouse IL‑6 (Catalog # 406-ML) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑6 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑6 R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1830). The ND₅₀ is typically 0.2-1 µg/mL.