| 货号 | AF1754-SP |
| 别名 | CD49 antigen-like family member E; CD49e antigen; CD49e; Fibronectin receptor subunit alpha; fibronectin receptor, alpha subunit; FNRAVLA5A; integrin alpha-5; Integrin alpha-F; integrin, alpha 5 (fibronectin receptor, alpha polypeptide); ITGA5; very late activation protein 5, alpha subunit; VLA-5 alpha; VLA-5 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/106cells) |
| 目标/特异性 | Detects mouse Integrin alpha 5/CD49e in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant human (rh) Integrin alpha 5 is observed, 15% cross‑reactivity with rhIntegrin alpha 2 and rhIntegrin alpha M is observed, and 5% cross‑reactivity with rhIntegrin alpha E is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/106cells |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3678 (Human); 16402 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Mechanical stress-activated integrin alpha5beta1 induces opening of connexin 43 hemichannels. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Integrin alpha 5/CD49e Phe45-Asn (Gln68Glu) Accession # P11688 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Integrins are transmembrane proteins that mediate interactions between adhesion molecules on adjacent cells and/or the extracellular matrix (ECM). Integrins have diverse roles in several biological processes including cell migration during development and wound healing, cell differentiation, and apoptosis. Their activities can also regulate the metastatic and invasive potential of tumor cells. They exist as heterodimers consisting of alpha and beta subunits. Some alpha and beta subunits exhibit specificity for one another and may be designated as a VLA (very late antigen) member. Heterodimers often preferentially bind certain cell adhesion molecules, or constituents of the ECM. Although they have no catalytic activity, integrins can be part of multimolecular signaling complexes known as focal adhesions. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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