Mouse Integrin alpha 2 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Mouse Integrin alpha 2 Affinity Purified Polyclonal Ab (25 UG)

货号： AF1740-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF1740-SP
别名	alpha-2 subunit; CD49b antigen; CD49b; integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor); ITGA2; VLA 2; VLA-2 alpha
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Flow Cytometry(0.25 µg/10⁶cells) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects mouse Integrin alpha 2/CD49b in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross‑reactivity with recombinant human Integrin alpha M is observed and less than 1% cross-reactivity with recombinant mouse (rm) Integrin alpha 5 and rmIntegrin alpha E is observed.
使用方法	Western Blot: 0.1 µg/mL Flow Cytometry: 0.25 µg/10⁶cells Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3673 (Human); 16398 (Mouse)
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse Integrin alpha 2/CD49b Tyr27-Thr1129 Accession # Q62469
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	The Integrin family proteins are heterodimeric transmembrane receptors comprised of an alpha and a beta subunit. The Integrin alpha 2 subunit is expressed in monocytes, platelets, B, T and NK cells. It forms a non-covalent heterodimer with the Integrin beta 1 subunit. Integrin alpha 2/ beta 1 binds collagen and laminin and activates intracellular signaling pathways.
运输条件	Blue Ice
存放说明	4℃
参考文献	Actin Stress Fibers and Focal Adhesions During EMT Adhesion Molecules Endothelial Cell Markers and Adhesion Molecules in Angiogenesis Epithelial Cell Polarity HGF/SF Activation of Integrins Human CD Marker Products ICC / IHC Images: Integrin alpha 2/CD49b Integrin Ligands at a Glance (J. Cell. Sci.) Integrin Subunit Interactions Integrins and Associated Molecules Isolation, Culture, and Identification of Natural Killer Cells Natural Killer T (NKT) Cells Platelets

参考图片

Detection of Integrin alpha 2/CD49b in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Mouse Anti-Mouse CD161/NK1.1 PE‑conjugated Monoclonal Antibody (Catalog # FAB8319P) and either (A) Sheep Anti-Mouse Integrin alpha 2/CD49b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1740) or (B) Normal Sheep IgG Control (Catalog # 5-001-A) followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127).

Integrin alpha 2/CD49b in Mouse Splenocytes. Integrin alpha 2/CD49b was detected in immersion fixed mouse splenocytes using Sheep Anti-Mouse Integrin alpha 2/CD49b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1740) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.