| 货号 | AF1639-SP |
| 别名 | CLEC5A; CLECSF5; CLECSF5carbohydrate-recognition domain) lectin, superfamilymember 5; C-type lectin domain family 5, member A; C-type lectin superfamily member 5; MDL-1MGC138304; Myeloid DAP12-associating lectin 1; myeloid DAP12-associating lectin-1 | 全称 | Myeloid DAP12-associating Lectin |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse MDL-1/CLEC5A in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human MDL-1/CLEC5A is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 23601 (Human); 23845 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Expression and functional role of MDL-1 (CLEC5A) in mouse myeloid lineage cells. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse MDL-1/CLEC5A Tyr26-Lys190 Accession # AAI04365 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | MDL-1 is anapproximately 40 kDa transmembrane glycoprotein belonging to the C-type lectinsuperfamily (CLEC5A). MDL-1 is expressed on immature myeloid cells, monocytes,macrophages, dendritic cells, neutrophils, NK cells, and osteocytes. Itcontains a charged lysine in the transmembrane region that enables it toassociate with DAP12 and deliver an activating signal. MDL-1 mediates inflammatory responses duringautoimmune arthritis and upon binding to Dengue and Japanese encephalitisviruses. The extracellular domain (ECD) of MDL-1 contains a juxtamembrane stalkregion and one C-type lectin domain. Within the ECD, mouse and human MDL-1share 67% amino acid sequence identity. In mouse, alternative splicinggenerates a short isoform with a 25 aa deletion in the stalk region. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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MDL‑1/CLEC5A in RAW 264.7 Mouse Cell Line. MDL‑1/CLEC5A was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Goat Anti-Mouse MDL‑1/CLEC5A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1639) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI(blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
