| 货号 | AF1620-SP |
| 别名 | Int-1; INT1Proto-oncogene Int-1 homolog; proto-oncogene Wnt-1; wingless-type MMTV integration site family, member 1 (oncogene INT1); wingless-type MMTV integration site family, member 1 | 全称 | Wingless-type MMTV Integration Site Family, Member 1 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse Wnt-1 in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant mouse (rm) Wnt‑3a and rmWnt‑5a is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7471 (Human); 22408 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Calmodulin-dependent protein kinase II/cAMP response element-binding protein/Wnt/beta-catenin signaling cascade regulates angiotensin II-induced podocyte injury and albuminuria. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse Wnt‑1 peptide Ser52-Glu92 and Met234-Arg313 Accession # Q3UR96 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Wnt-1 is a secreted glycoprotein that binds members of the frizzledfamily of seven transmembrane domain receptors. Wnt-1 may have animportant role in the morphogenesis of the neural tube and in CNSdevelopment. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Wnt‑1 in Mouse Embryonic Spinal Cord. Wnt‑1 was detected in immersion fixed frozen sections of mouse embryonic spinal cord (13 d.p.c.) using 15 µg/mL Goat Anti-Mouse Wnt‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1620) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
