Mouse SOST/Sclerostin Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse SOST/Sclerostin Affinity Purified Polyclonal Ab (25 UG)

货号： AF1589-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF1589-SP
别名	sclerostin; VBCHsclerosteosis
反应种属	Mouse
应用	Western Blot(2 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects mouse SOST/Sclerostin in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human SOST is observed.
使用方法	Western Blot: 2 µg/mL Immunohistochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	50964 (Human); 74499 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Distorted Patterns of Dentinogenesis and Eruption in Msx2 Null Mutants: Involvement of Sost/Sclerostin Am J Pathol, 2016;0(0):. Species: Mouse Sample Type: Whole Tissue Application: IHC - Paraffin embedded Parathyroid Hormone Induces Bone Cell Motility and Loss of Mature Osteocyte Phenotype through L-Calcium Channel Dependent and Independent Mechanisms. Authors: Prideaux M, Dallas S, Zhao N, Johnsrud E, Veno P, Guo D, Mishina Y, Harris S, Bonewald L PLoS ONE, 2015;10(5):e0125731. Species: Mouse Sample Type: Cell Lysates Application: WB Heavy Metal Ion Regulation of Gene Expression: MECHANISMS BY WHICH LEAD INHIBITS OSTEOBLASTIC BONE-FORMING ACTIVITY THROUGH MODULATION OF THE Wnt/beta-CATENIN SIGNALING PATHWAY. Authors: Beier E, Sheu T, Dang D, Holz J, Ubayawardena R, Babij P, Puzas J J Biol Chem, 2015;290(29):18216-26. Species: Mouse Sample Type: Cell Lysates Application: WB The Wnt Inhibitor Sclerostin Is Up-regulated by Mechanical Unloading in Osteocytes in Vitro. Authors: Spatz J, Wein M, Gooi J, Qu Y, Garr J, Liu S, Barry K, Uda Y, Lai F, Dedic C, Balcells-Camps M, Kronenberg H, Babij P, Pajevic P J Biol Chem, 2015;290(27):16744-58. Species: Mouse Sample Type: Cell Lysate Application: WB Osteocytes mediate the anabolic actions of canonical Wnt/beta-catenin signaling in bone. Authors: Tu X, Delgado-Calle J, Condon K, Maycas M, Zhang H, Carlesso N, Taketo M, Burr D, Plotkin L, Bellido T Proc Natl Acad Sci U S A, 2015;112(5):E478-86. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Inhibition of GSK-3beta rescues the impairments in bone formation and mechanical properties associated with fracture healing in osteoblast selective connexin 43 deficient mice. Authors: Loiselle A, Lloyd S, Paul E, Lewis G, Donahue H PLoS ONE, 2013;8(11):e81399. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded miR-218 directs a Wnt signaling circuit to promote differentiation of osteoblasts and osteomimicry of metastatic cancer cells. Authors: Hassan, Mohammad, Maeda, Yukiko, Taipaleenmaki, Hanna, Zhang, Weibing, Jafferji, Mohammad, Gordon, Jonathan, Li, Zhaoyong, Croce, Carlo M, van Wijnen, Andre J, Stein, Janet L, Stein, Gary S, Lian, Jane B J Biol Chem, 2012;287(50):42084-92. Species: Mouse Sample Type: Cell Lysates Application: WB Repression of osteocyte Wnt/ÃŽÂ²-catenin signaling is an early event in the progression of renal osteodystrophy. Authors: Sabbagh Y, Graciolli FG, OBrien S, Tang W, Dos Reis LM, Ryan S, Phillips L, Boulanger J, Song W, Bracken C, Liu S, Ledbetter S, Dechow P, Canziani ME, Carvalho AB, Jorgetti V, Moyses RM, Schiavi SC J. Bone Miner. Res., 2012;27(8):1757-72. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Matrix metalloproteinase-13 is required for osteocytic perilacunar remodeling and maintains bone fracture resistance. J. Bone Miner. Res., 2012;27(9):1936-50. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Conditional deletion of Bmpr1a in differentiated osteoclasts increases osteoblastic bone formation, increasing volume of remodeling bone in mice. Authors: Okamoto M, Murai J, Imai Y, Ikegami D, Kamiya N, Kato S, Mishina Y, Yoshikawa H, Tsumaki N J. Bone Miner. Res., 2011;26(10):2511-22. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Parathyroid hormone receptor signaling in osteocytes increases the expression of fibroblast growth factor-23 in vitro and in vivo. Authors: Rhee Y, Bivi N, Farrow E, Lezcano V, Plotkin LI, White KE, Bellido T Bone, 2011;49(4):636-43. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded The Appearance and Modulation of Osteocyte Marker Expression during Calcification of Vascular Smooth Muscle Cells. Authors: Zhu D, Mackenzie NC, Millan JL, Farquharson C, MacRae VE PLoS ONE, 2011;6(5):e19595. Species: Mouse Sample Type: Cell Lysates Application: WB Distinct modes of inhibition by sclerostin on bone morphogenetic protein and Wnt signaling pathways. Authors: Krause C, Korchynskyi O, de Rooij K, Weidauer SE, de Gorter DJ, van Bezooijen RL, Hatsell S, Economides AN, Mueller TD, Lowik CW, ten Dijke P J. Biol. Chem., 2010;285(53):41614-26. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Localization of SOST/sclerostin in cementocytes in vivo and in mineralizing periodontal ligament cells in vitro. Authors: Jager A, Gotz W, Lossdorfer S, Rath-Deschner B J. Periodont. Res., 2010;45(2):246-54. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Parathyroid hormone signaling through low-density lipoprotein-related protein 6. Authors: Wan M, Yang C, Li J, Wu X, Yuan H, Ma H, He X, Nie S, Chang C, Cao X Genes Dev., 2008;22(21):2968-79. Species: Mouse Sample Type: Whole Tissue Application: IHC Fresh Control of the SOST bone enhancer by PTH using MEF2 transcription factors. Authors: Leupin O, Kramer I, Collette NM, Loots GG, Natt F, Kneissel M, Keller H J. Bone Miner. Res., 2007;22(12):1957-67. Species: Rat Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse SOST/Sclerostin Gln24-Tyr211 Accession # NP_077769
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	SOST, also known as sclerostin, is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. Cerberus/DAN family members are putative BMP antagonists, and include Dan, Cerberus, Gremlin, PRDC, and Caronte. While the overall sequence identity between members of the family is low, they have conserved spacing of six cysteine residues. Cerberus and Dan have an additional cysteine residue used for dimerization; however, SOST does not and is secreted as a monomer. SOST was originally identified as an important regulator of bone homeostasis. Positional cloning studies identified that mutations in the SOST gene can cause sclerosteosis and van Buchem disease, bone dysplasia disorders characterized by progressive skeletal overgrowth. Significant levels of SOST expression are detected in bone, cartilage, kidney, and liver. SOST is expressed by osteoclasts in developing bones of mouse embryos, including both intramembranously forming skull bones and endochondrally forming long bones. SOST plays a physiological role as a negative regulator of bone formation by repressing BMP-induced osteogenesis. SOST has been shown to have unique ligand specificity, binding BMP-5, -6, and -7 with high affinity and BMP-2 and -4 with low affinity. This seems to be the first example of a BMP antagonist being localized to osteoclasts, cells derived from the hematopoietic lineage, that function to degrade bone matrix. Human and mouse SOST share 88% amino acid identity (1-3).
运输条件	Blue Ice
存放说明	4℃
参考文献	Kusu, N. et al. (2003) J. Biol. Chem. 278:24113. Balemans, W. et al. (2001) Hum. Mol. Genet. 10:537. Brunkow, M.E. et al. (2001) Am. J. Hum. Genet. 68:577.

参考图片

Detection of Human and Mouse SOST/Sclerostin by Western Blot. Western blot shows lysates of human bone marrow and mouse bone marrow. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse SOST/Sclerostin Antigen Affinity-purified Polyclonal Antibody
(Catalog # AF1589) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SOST/Sclerostin at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

SOST/Sclerostin in Mouse Embryo. SOST/Sclerostin was detected in immersion fixed frozen sections of mouse embryo (kidney) using Goat Anti-Mouse SOST/Sclerostin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1589) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to glomeruli. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

SOST/Sclerostin in Mouse Embryo. SOST/Sclerostin was detected in immersion fixed frozen sections of mouse embryo (adrenal gland) using Goat Anti-Mouse SOST/Sclerostin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1589) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

SOST/Sclerostin in Mouse Embryo. SOST/Sclerostin was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse SOST/Sclerostin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1589) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.