货号 | AF1476-SP |
别名 | EFABP; E-FABP; E-FABPPAFABP; fatty acid binding protein 5 (psoriasis-associated); Fatty acid-binding protein 5; PA-FABPepidermal; Psoriasis-associated fatty acid-binding protein homolog | 全称 | Fatty Acid-Binding Protein 5 |
反应种属 | Mouse |
应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
目标/特异性 | Detects mouse FABP5/E‑FABP in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant mouse FABP4 and recombinant human (rh) FABP3 is observed and less than 1% cross-reactivity with recombinant rat (rr) FABP2 and rrFABP1 is observed. |
使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL |
来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 2171 (Human); 16592 (Mouse) |
应用文献 | |
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. BLIMP1 is required for postnatal epidermal homeostasis but does not define a sebaceous gland progenitor under steady-state conditions. | |
纯化方式 | Antigen Affinity-purified |
免疫原 | E. coli-derived recombinant mouse FABP5/E‑FABP Met1-Gln135 Accession # Q05816 |
生物活性 | Mouse |
标记 | Unconjugated |
溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
背景 | Mouse FABP5, also named Keratinocyte lipid-binding protein (KLBP) andMal-1, is a member of the intracellular FABP family. It is expressedmost abundantly in keratinocytes but is also found in differentiatedadipocytes, macrophages and stratified epithelia of epidermis andtongue. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of Human, Mouse, and Rat FABP5/E‑FABP by Western Blot. Western blot shows lysates of human placenta tissue, mouse liver tissue, rat brain tissue, and rat heart tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/ Rat FABP5/E‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1476) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FABP5/ E‑FABP at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |