| 货号 | AF1443-SP |
| 别名 | Adipocyte lipid-binding protein; Adipocyte-type fatty acid-binding protein; A-FABP; A-FABPAFABP; ALBP; aP2; fatty acid binding protein 4, adipocyte; Fatty acid-binding protein 4; fatty acid-binding protein, adipocyte | 全称 | Fatty Acid-Binding Protein 4 |
| 反应种属 | Mouse/Rat |
| 应用 | Western Blot(0.25 µg/mL) Simple Western(2.5 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse FABP4 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) FABP5 and rmFABP9 is observed. |
| 使用方法 | Western Blot: 0.25 µg/mL Simple Western: 2.5 µg/mL Immunohistochemistry: 5-15 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2167 (Human); 11770 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Artificial sweeteners stimulate adipogenesis and suppress lipolysis independently of sweet taste receptors. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse FABP4 Met1-Ala132 Accession # P04117 |
| 生物活性 | Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | FABP-4 (fatty acid binding protein-4; also Adipocyte-type FABP, ALBP and aP2) is a 14-15 kDa member of the fatty acid binding protein family, calycin superfamily of molecules. It is expressed by adipocytes, macrophages, monocyte-derived DC and myoepithelial cells, and is known to serve as a carrier for select PPAR gamma fatty acid ligands as they enter the nucleus. These ligands, or fatty acids, facilitate PPAR gamma -mediated gene transcription. Mouse FABP-4 is 132 amino acids (aa) in length. It is a two beta -sheet molecule that contains an NLS (aa 22-32) and a fatty acid binding segment (aa 127-129). Mouse FABP-4 is 94% and 92% aa identical to rat and human FABP-4, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse FABP4/A‑FABP by Western Blot. Western blot shows lysates of mouse thymus tissue and mouse heart tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse/Rat FABP4/A‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for FABP4/A‑FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
FABP4 in Mouse Adipocytes. FABP4 was detected in immersion fixed mouse mesenchymal stem cell-derived adipocytes using 10 µg/mL Goat Anti-Mouse/Rat FABP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
FABP4 in Rat Mesenchymal Stem Cells. FABP4 was detected in immersion fixed rat mesenchymal stem cells differentiated to adipocytes using Goat Anti-Mouse/Rat FABP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
FABP4 in Mouse Intestine. FABP4 was detected in perfusion fixed frozen sections of mouse intestine using Goat Anti-Mouse/Rat FABP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells in intestinal glands. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. | |
Detection of Mouse FABP4/A‑FABP by Simple WesternTM. Simple Western lane view shows lysates of mouse thymus tissue and mouse heart tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP4/A‑FABP at approximately 17 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse/Rat FABP4/A‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog #HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
