| 货号 | AF1436-SP |
| 别名 | Igfals | 全称 | IGF-binding Protein Acid Labile Subunit |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.25 µg/mL) |
| 目标/特异性 | Detects mouse ALS in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.25 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 16005 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Early postnatal nutrition determines somatotropic function in mice. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse ALS Thr28-Cys603 Accession # P70389 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | ALS (Acid labile subunit) is an 84-86 kDa glycoprotein member of the leucine rich repeat superfamily of molecules. It is secreted by hepatocytes in response to growth hormone stimulation, and forms a 140-150 kDa ternary complex with IGFBP3 (or IGFBP5) and IGF-I. This complex increases the half-life of IGF-I and retains it in the circulation for use in multiple tissues. Mature mouse ALS is 580 amino acids (aa) in length (aa 24-603). It contains twenty-one 20-23 aa LRRs that participate in protein-protein interactions. There are two potential isoform variants that show N-terminal extensions. One contains a 66 aa substitution for aa 1-5, while another possesses an 89 aa substitution again for aa 1-5. Over aa 28-603, mouse ALS shares 79% and 93% aa identity with human and rat ALS, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Mouse ALS by Western Blot. Western blot shows lysates of mouse spleen tissue, mouse liver tissue, and mouse bone marrow. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse ALS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1436) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ALS at approximately 80-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
