| 货号 | AF135-SP |
| 别名 | WIF-1; WNT inhibitory factor 1 | 全称 | Wnt Inhibitory Factor |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects mouse WIF-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human WIF-1 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 11197 (Human); 24117 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Wnt inhibitory factor (WIF)-1 inhibits osteoblastic differentiation in mouse embryonic mesenchymal cells. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse WIF-1 Gly29-Trp379 Accession # Q9WUA1 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | WIF-1 (Wnt Inhibitory Factor 1) is a secreted protein that binds to Wnt proteins and inhibits their activity. In situ hybridization analysis in Xenopus laevis and zebrafish indicate that the message is highly expressed in presomitic mesoderm, the notochord, anterior regions of the brain, branchial arches, nasal placodes, and otic vescicles. WIF-1 inhibits secondary axis induction by Wnts and promotes secondary axis induction by Chordin in Xenopus embryos. In vitro, WIF-1 binds to Drosophila Wingless and Xenopus Wnt8 proteins. WIF-1 is implicated as an early event tumor suppressor in cancers of the prostate, breast, lung, and bladder, while in other cancer types, such as colon adenocarcinoma, WIF-1 facilitates tumorigenesis. WIF-1 is also expressed in mammalian retina and may play a role in photoreceptor development. The protein is synthesized as a 379 amino acid (aa) molecule that contains a signal sequence, a 150 aa WIF domain, 5 EGF-like repeats, and a hydrophilic domain at the carboxy terminus. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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