| 货号 | AF1357-SP |
| 别名 | BR3; CD268; TNFRSF13C | 全称 | B cell Activating Factor Receptor |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) ELISA Capture (Matched Antibody Pair)(0.2-0.8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( ) |
| 目标/特异性 | Detects mouse BAFF R in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human BAFF R is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Blockade of Receptor-ligand Interaction: In a functional ELISA, 0.1-0.4 µg/mL of this antibody will block 50% of the binding of 100 ng/mL of Recombinant Mouse BAFF/BLyS/TNFSF13B (Catalog # 2106-BF) to immobilized Recombinant Mouse BAFF R/TNFRSF13C Fc Chimera (Catalog # 1357-BR) coated at 200 ng/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding. ELISA Capture (Matched Antibody Pair): 0.2-0.8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 115650 (Human); 72049 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Functional implication of BAFF synthesis and release in gangliosides-stimulated microglia. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse BAFF R Ser10-Ala71 Accession # Q9D8D0 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | B-cell activating factor (BAFF), also known as BlyS, TALL-1, TNAK, and zTNF4, is a TNF ligand superfamily member and has been designated TNFSF13B. It is produced by macrophages, dendritic cells, and T lymphocytes. BAFF promotes the survival of B cells and is essential for B cell maturation (1 - 4). BAFF binds to three TNF receptor superfamily members: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF 13C). These receptors are type III transmembrane proteins that lack a signal peptide. Whereas TACI and BCMA bind BAFF and another TNF superfamily ligand, APRIL(a proliferation-inducing ligand), BAFF-R selectively binds BAFF. Mouse BAFF-R cDNA encodes a 175 amino acid residue (aa) transmembrane protein with a 71 aa extracellular domain, a 21 aa transmembrane domain, and a 83 aa cytoplasmic region. A second isoform of BAFF R that has a 72 aa cytoplamic region can also be produced by alternative splicing. The BAFF R extracellular domain lacks the TNF receptor canonical cysteine-rich domain (CRD) and contains only a partial CRD with four cysteine residues. Human and mouse BAFF R share 56% aa sequence identity. BAFF R is highly expressed in spleen, lymph node and resting B cells. It is also expressed at lower levels in activated B cells, in resting CD4+T cells, in thymus and peripheral blood leukocytes. BAFF knockout mice lack mature B cells and has profound defects in antibody mediated immune responses. Similarly, A/WySnJ mice that are defective in BAFF R intracellular signaling also lack mature B cells, suggesting that BAFF R is the critical receptor for BAFF during B lymphopoiesis. In contrast, BCMA- or TACI-deficient mice have no major defect in B-cell development. While the function of BCMA is not defined, TACI has been shown to control B-cell homeostasis and T-cell-independent immune responses. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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