| 货号 | AF1307-SP |
| 别名 | frizzled (Drosophila) homolog 2; frizzled homolog 2 (Drosophila); frizzled-2; Fz2; fz-2; FZD2; fzE2; hFz2 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects mouse Frizzled-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant mouse (rm) Frizzled-1 is observed and less than 5% cross-reactivity with rmFrizzled-4, recombinant human Frizzled-5, rmFrizzled-6, and rmFrizzled-8 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2535 (Human); 57265 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Wnt-5a/Frizzled9 receptor signaling through the G?o/G?? complex regulates dendritic spine formation | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-2 Gln29-Leu168 Accession # Q9JIP6 |
| 内毒素水平 | <0.50 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Wnt signaling is involved in variety of developmental processes including cell fate determination, cell polarity, tissue patterning and control of cell proliferation. Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway. The founding member of this family was identified in Drosophila based on its role in tissue polarity in the adult cuticle and named for the disorganized appearance of bristle hairs on the mutant. Ten mammalian Frizzled genes have been identified to date. The predicted structure of Frizzled proteins is similar among all family members, containing a divergent N-terminal signal peptide, a highly conserved extracellular cysteine-rich domain, a variable-length linker region, a seven-pass transmembrane domain, and a variable-length C-terminal tail. One of the most conserved regions of the Frizzled proteins is the extracellular cysteine-rich domain (CRD) which spans approximately 120 AA and contains 10 invariant cysteines (1). Mouse Frizzled-2 shows 100% amino acid identity to human and rat Frizzled-2 in the CRD region. Frizzled-2 expression is greater in embryonic than adult tissues, with heart, brain, lung, kidney and gut showing the highest levels (2). In addition, Frizzled-2 is expressed during migration and proliferation of neural crest cells that populate the heart and great arteries (3). However, Frizzled-2 may not be strictly involved in developmental processes, as its expression is also upregulated in myofibroblasts during tissue repair after myocardial infarction (4). Frizzled-2 is implicated in the Wnt/Ca2+ pathway, a mechanism by which Wnt-5a signaling results in calcium release from intracellular stores and activation of PKC and calmodulin-dependent protein kinase (5). Secreted frizzled related proteins (sFRPs), antagonists of the Wnt pathway, have been shown to interact with both Wnt ligands and Frizzled receptors (6). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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