货号 | AF1279-SP |
别名 | CD72 antigenB-cell differentiation antigen CD72; CD72 molecule; CD72; CD72b; Ly-19.2; Ly-32.2; Lyb-2; LYB2lyb-2 |
反应种属 | Mouse |
应用 | Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
目标/特异性 | Detects mouse CD72 in direct ELISAs and Western blots. In Western blots, less than 1% cross-reactivity with recombinant human CD72 is observed. |
使用方法 | Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL |
来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 971 (Human); 12517 (Mouse) |
纯化方式 | Antigen Affinity-purified |
免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse CD72 Arg117-Asn354 Accession # P21855 |
生物活性 | Mouse |
标记 | Unconjugated |
溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
背景 | CD72, also known as Lyb2, is a transmembrane lectin-like inhibitory receptor expressed on B lineage cells. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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CD72 in Mouse Splenocytes. CD72 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse CD72 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1279) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |