| 货号 | AF122-SP |
| 别名 | IL18 BPd; IL-18 BPd | 全称 | Interleukin 18 Binding Protein Isoform d |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects mouse IL‑18 BPd in direct ELISAs and Western blots. In Western blots, approximately 25% cross‑reactivity with recombinant mouse IL-18 BPc is observed and less than 2% cross-reactivity with recombinant human IL-18 BPa is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize IL‑18 BPd inhibition of IL‑18/IL‑1F4-induced IFN‑ gamma secretion in activated mouse T cells. The Neutralization Dose (ND50) is typically 10-30 µg/mL in the presence of 2 µg/mL Recombinant Mouse IL‑18 BPd Fc Chimera, 0.5 ng/mL Recombinant Mouse IL‑18/IL‑1F4, and 0.1 ng/mL Recombinant Mouse IL‑12. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10068 (Human); 16068 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Correction: Yersinia pestis Activates Both IL-1beta and IL-1 Receptor Antagonist to Modulate Lung Inflammation during Pneumonic Plague. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse IL‑18 BPd Thr27-Ala191 Accession # AAD17194 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Interleukin 18 binding protein (IL-18 BP) is a secreted glycoprotein, which functions as an IL-18 antagonist by binding to IL-18 and blocking its biological activity. IL‑18 BP bears no amino acid sequence homology to the membrane-associated IL-18 and IL-1 receptor proteins. The gene for human IL-18 BP has been localized to chromosome 11q13. It encodes for at least four isoforms by alternative splicing. The IL-18 BP isoforms a and c each contain one immunoglobulin (Ig)-like C2-type domain while isoforms b and d lack a complete Ig domain. The complete Ig domain has been shown to be essential to the binding and neutralizing properties of the binding proteins. Two isoforms of mouse IL18 BP (c and d) containing the complete Ig domain have also been isolated and shown to neutralize IL-18 bioactivity. Human and mouse IL-18 BPs share approximately 61% amino acid sequence identity. Several poxviruses also encode proteins with sequence similarity to the human and mouse IL-18 BP. Viral IL-18 BPs have been shown to bind and inhibit IL-18 responses and may be involved in modulating host immune responses. The expression of IL-18 BP is markedly up-regulated by IFN-gamma, suggesting that IL-18 activity is modulated by a negative feedback mechanism mediated by IL-18 BP. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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IL‑18 BPd Inhibition of IL‑18/IL‑1F4-induced IFN‑ gamma Secretion and Neutralization by Mouse IL‑18 BPd Antibody. Recombinant Mouse IL‑18 BPd Fc Chimera (Catalog # 122-BP) inhibits Recombinant Mouse IL‑18/IL‑1F4 induced IFN‑ gamma secretion in activated mouse T cells in a dose-dependent manner (orange line), as measured by the Mouse IFN‑ gamma Quantikine ELISA Kit (Catalog # MIF00). Inhibition of Recombinant Mouse IL‑18/ IL‑1F4 (0.5 ng/mL) activity elicited by Recombinant Mouse IL‑18 BPd Fc Chimera (2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑18 BPd Antigen Affinity-purified Polyclonal Antibody (Catalog # AF122). The ND50 is typically 10-30 µg/mL in the presence of Recombinant Mouse IL‑12 (0.1 ng/mL). |
