Mouse Sca-1 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse Sca-1 Affinity Purified Polyclonal Ab (25 UG)

货号： AF1226-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF1226-SP
别名	Ly6
全称	Stem Cell Antigen-1/Lymphocyte Antigen 6
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects mouse Sca-1/Ly6 in direct ELISAs and Western blots.
使用方法	Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	110454 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Pluripotent stem cells derived from mouse and human white mature adipocytes. Authors: Jumabay M, Abdmaulen R, Ly A, Cubberly M, Shahmirian L, Heydarkhan-Hagvall S, Dumesic D, Yao Y, Bostrom K Stem Cells Transl Med, 2014;3(2):161-71. Species: Mouse Sample Type: Whole Cells Application: ICC Age dependence of lung mesenchymal stromal cell dynamics following pneumonectomy. Authors: Paxson J, Gruntman A, Davis A, Parkin C, Ingenito E, Hoffman A Stem Cells Dev, 2013;22(24):3214-25. Species: Mouse Sample Type: tissue Application: IHC Paraffin-embedded Distinct fibroblast lineages determine dermal architecture in skin development and repair. Authors: Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F Nature, 2013;504(7479):277-81. Species: Mouse Sample Type: tissue Application: IHC Frozen Stromal cell-derived factor-1 enhances distraction osteogenesis-mediated skeletal tissue regeneration through the recruitment of endothelial precursors. Authors: Fujio M, Yamamoto A, Ando Y, Shohara R, Kinoshita K, Kaneko T, Hibi H, Ueda M Bone, 2011;49(4):693-700. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Three-dimensional culture of mouse renal carcinoma cells in agarose macrobeads selects for a subpopulation of cells with cancer stem cell or cancer progenitor properties. Authors: Smith BH, Gazda LS, Conn BL Cancer Res., 2011;71(3):716-24. Species: Mouse Sample Type: Whole Tissue Application: IHC Structural basis of digoxin that antagonizes RORgamma t receptor activity and suppresses Th17 cell differentiation and interleukin (IL)-17 production. Authors: Fujita-Sato S, Ito S, Isobe T, Ohyama T, Wakabayashi K, Morishita K, Ando O, Isono F J. Biol. Chem., 2011;286(36):31409-17. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Prominin-1/CD133+ lung epithelial progenitors protect from bleomycin-induced pulmonary fibrosis. Authors: Germano D, Blyszczuk P, Valaperti A, Kania G, Dirnhofer S, Landmesser U, Luscher TF, Hunziker L, Zulewski H, Eriksson U Am. J. Respir. Crit. Care Med., 2009;179(10):939-49. Species: Mouse Sample Type: Whole Cells Application: Flow A Gata6-Wnt pathway required for epithelial stem cell development and airway regeneration. Authors: Zhang Y, Goss AM, Cohen ED, Kadzik R, Lepore JJ, Muthukumaraswamy K, Yang J, DeMayo FJ, Whitsett JA, Parmacek MS, Morrisey EE Nat. Genet., 2008;40(7):862-70. Species: Mouse Sample Type: Whole Cells Application: ICC Intestinal epithelial cell up-regulation of LY6 molecules during colitis results in enhanced chemokine secretion. Authors: Flanagan K, Modrusan Z, Cornelius J, Chavali A, Kasman I, Komuves L, Mo L, Diehl L J. Immunol., 2008;180(6):3874-81. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen The recruitment of primitive Lin(-) Sca-1(+), CD34(+), c-kit(+) and CD271(+) cells during the early intraperitoneal foreign body reaction. Authors: Vranken I, De Visscher G, Lebacq A, Verbeken E, Flameng W Biomaterials, 2007;29(7):797-808. Species: Rat Sample Type: Whole Cells Application: ICC Identification of pulmonary Oct-4+ stem/progenitor cells and demonstration of their susceptibility to SARS coronavirus (SARS-CoV) infection in vitro. Authors: Ling TY, Kuo MD, Li CL, Yu AL, Huang YH, Wu TJ, Lin YC, Chen SH, Yu J Proc. Natl. Acad. Sci. U.S.A., 2006;103(25):9530-5. Species: Mouse Sample Type: Whole Cells Application: ICC Identification of bronchioalveolar stem cells in normal lung and lung cancer. Authors: Kim CF, Jackson EL, Woolfenden AE, Lawrence S, Babar I, Vogel S, Crowley D, Bronson RT, Jacks T Cell, 2005;121(6):823-35. Species: Mouse Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse Sca-1 C-terminally truncated Ly-6E allele Leu27-Gly119 Accession # CAA28351
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	This antibody reacts with Sca-1, an 18 kDa phosphatidylinositol-anchored protein that is a member of the lymphocyte antigen 6 (Ly-6) family (1). Sca-1 is encoded by the strain-specific Ly-6 E/A allelic gene. Its expression on multipotent hematopoietic stem cells (HSC) has been used as a marker of HSC in mice of both Ly‑6 haplotypes (2, 3). This antibody is frequently used in combination with lineage depletion antibodies to identify and isolate murine HSC. Sca-1-positive HSC can be found in the adult bone marrow, fetal liver and mobilized peripheral blood and spleen in the adult animal (2‑7). However, Sca-1 has also been discovered in several non‑hematopoietic tissues (1) and can be used to enrich progenitor cell populations other than HSC (8). It is suggested that Sca-1 could be involved in regulating both B and T cell activation (9‑12).
运输条件	Blue Ice
存放说明	4℃
参考文献	Van de Rijn, M. et al. (1989) Proc. Natl.Acad. Sci. USA 86:4634. Spangrude, G.I. et al. (1988) Science 241:58. Spangrude, G.I. et al. (1993) Blood 82:3327. Morrison, S.J. et al. (1995) Proc. Natl. Acad. Sci. USA 92:10302. Kawamoto, H. et al. (1997) Int. Immunol. 9:1011. Yamamoto, Y. et al. (1996) Blood 88:445. Morrison, S.J. et al. (1997) Proc. Natl. Acad. Sci. USA 94:1908. Welm, B.E. et al. (2002) Dev. Biol. 245:42. Codias, E.K. et al. (1990) J. Immunol. 144:2197. Malek, T.R. et al. (1986) J. Exp. Med. 164:709 . Codias, E.K. et al. (1990) J. Immunol. 145:1407. Flood, P.M. et al. (1990) J. Exp. Med. 172:115.

参考图片

Sca‑1/Ly6 in Mouse Splenocytes. Sca‑1/Ly6 was detected in immersion fixed mouse splenocytes using 10 µg/mL Goat Anti-Mouse Sca‑1/Ly6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1226) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.