Mouse/Rat/Canine ALCAM Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse/Rat/Canine ALCAM Affinity Purified Polyclonal Ab (25 UG)

货号： AF1172-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF1172-SP
别名	activated leucocyte cell adhesion molecule; activated leukocyte cell adhesion moleculeFLJ38514; CD166 antigen; CD166; MEMDMGC71733
全称	Activated Leukocyte Cell Adhesion Molecule
反应种属	Mouse/Rat/Canine
应用	Western Blot(0.25 µg/mL) Simple Western(12.5 µg/mL) Flow Cytometry(0.25 µg/10⁶cells) Immunohistochemistry(5-15 µg/mL) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects mouse ALCAM in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human (rh) ALCAM is observed, and less than 10% cross-reactivity with rhBCAM, recombinant mouse (rm) OCAM, and rmMAdCAM‑1 is observed.
使用方法	Western Blot: 0.25 µg/mL Simple Western: 12.5 µg/mL Flow Cytometry: 0.25 µg/10⁶cells Immunohistochemistry: 5-15 µg/mL Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	214 (Human); 11658 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Transcriptome analysis reveals transmembrane targets on transplantable midbrain dopamine progenitors. Authors: Bye C, Jonsson M, Bjorklund A, Parish C, Thompson L Proc Natl Acad Sci U S A, 2015;112(15):E1946-55. Species: Mouse Sample Type: Whole Tissue Application: IHC - Not specified Ionizing irradiation induces acute haematopoietic syndrome and gastrointestinal syndrome independently in mice. Authors: Leibowitz B, Wei L, Zhang L, Ping X, Epperly M, Greenberger J, Cheng T, Yu J Nat Commun, 2014;5(0):3494. Species: Mouse Sample Type: Whole Tissue Application: IF Cocaine hijacks sigma1 receptor to initiate induction of activated leukocyte cell adhesion molecule: implication for increased monocyte adhesion and migration in the CNS. Authors: Yao H, Kim K, Duan M, Hayashi T, Guo M, Morgello S, Prat A, Wang J, Su TP, Buch S J. Neurosci., 2011;31(16):5942-55. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Molecular mechanisms controlling midline crossing by precerebellar neurons. Authors: Di Meglio T, Nguyen-Ba-Charvet KT, Tessier-Lavigne M, Sotelo C, Chedotal A J. Neurosci., 2008;28(25):6285-94. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Inhibition of p38 MAPK signaling in chondrocyte cultures results in enhanced osteogenic differentiation of perichondral cells. Authors: Stanton LA, Beier F Exp. Cell Res., 2007;313(1):146-55. Species: Mouse Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse ALCAM/CD166 Trp28-Lys527 Accession # AAC06342
生物活性	Mouse, Rat, Canine
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	ALCAM, activated leukocyte cell adhesion molecule, is a type I membrane glycoprotein and a member of the immunoglobulin supergene family. It is also known as CD166, MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. ALCAM is expressed on thymic epithelial cells, activated B and T cells, and monocytes. ALCAM can bind itself homotypically and is also capable of binding CD6, NgCAM, and other, as of yet, unidentified brain proteins. ALCAM/CD6 interaction may be involved in T cell development and T cell regulation. Additionally, ALCAM/CD6 and ALCAM/NgCAM interactions may play roles in the nervous system. ALCAM has also been observed to be upregulated on highly metastasizing melanoma cell lines and may play a role in tumor migration. ALCAM is a 583 amino acid (aa) protein consisting of a 27 aa signal peptide, a 500 aa extracellular domain, a 24 aa transmembrane domain, and a 32 aa cytoplasmic domain. The extracellular domain of ALCAM contains 5 Ig-like domains of which the amino-terminal V1 domain is essential for ligand binding and ALCAM-mediated cell aggregation (1-4). The ECD of mouse ALCAM shares 97.5% and 93.2% aa sequence identity with rat and canine ALCAM ECD, respectively.
运输条件	Blue Ice
存放说明	4℃
参考文献	Bowen, M.A.et al. (1995) J. Exp. Med. 181:2213. Aruffo, A.et al. (1997) Immunol. Today 18:498. Degen, W.G.et al. (1998) Am. J. Pathol. 152:805. Van Kempen, L. et al. (2001) J. Biol. Chem. 276:25783.

参考图片

Detection of Mouse ALCAM/CD166 by Western Blot. Western blot shows lysates of mouse brain (total) tissue, mouse brain (cerebellum) tissue, and mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse/Rat/Canine ALCAM/CD166 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1172) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ALCAM/CD166 at approximately 90-120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of ALCAM/CD166 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either (A) activated or (B) resting were stained with Goat Anti-Mouse/Rat/Canine ALCAM/CD166 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1172) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107) and Rat Anti-Mouse B220/CD45R APC‑conjugated Monoclonal Antibody (Catalog # FAB1217A). Quadrant markers were set based on control antibody staining
(Catalog #AB-108-C).

Detection of ALCAM/CD166 in Canine PBMCs by Flow Cytometry. Canine peripheral blood mononuclear cells (PBMCs) treated with PMA and Calcium Ionomycin for 24 hours were stained with Goat Anti-Mouse/Rat/Canine ALCAM/CD166 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1172, filled histogram) or isotype control antibody (Catalog # AB‑108‑C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

ALCAM/CD166 in Mouse Liver.

ALCAM/CD166 was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse/Rat/Canine ALCAM/CD166 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1172) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

ALCAM/CD166 in Rat Mesenchymal Stem Cells. ALCAM/CD166 was detected in immersion fixed undifferentiated rat mesenchymal stem cells using Goat Anti-Mouse/Rat/Canine ALCAM/CD166 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1172) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Mouse ALCAM/CD166 by Simple Western^TM. Simple Western lane view shows lysates of mouse brain tissue and mouse lung tissue loaded at 0.2 mg/mL. A specific band was detected for ALCAM/CD166 at approximately 149 kDa
(as indicated) using 12.5 µg/mL of Goat Anti-Mouse/Rat/Canine ALCAM/CD166 Antigen Affinity-purified Polyclonal Antibody
(Catalog # AF1172) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.