| 货号 | AF1157-SP |
| 别名 | CD271 antigen; CD271; Gp80-LNGFR; member 16; nerve growth factor receptor; NGF receptor; p75 ICD; p75 NTR; TNFRSF16; TNFRSF16nerve growth factor receptor (TNFR superfamily, member 16); tumor necrosis factor receptor superfamily member 16 | 全称 | Nerve Growth Factor Receptor |
| 反应种属 | Mouse |
| 应用 | Western Blot(2 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse NGF R/TNFRSF16 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human NGF R/TNFRSF16 is observed. |
| 使用方法 | Western Blot: 2 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4804 (Human); 18053 (Mouse); 117089 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Superior Cervical Ganglia Neurons Induce Foxp3+ Regulatory T Cells via Calcitonin Gene-Related Peptide | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse NGF R/TNFRSF16 Gly20-Asn243 Accession # Q9Z0W1 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The low affinity nerve growth factor receptor (NGF R), also named p75 neurotrophin receptor, is a type I transmembrane protein that belongs to the tumor necrosis factor receptor family and has been designated TNFRSF16. NGF R cDNA encodes a 427 amino acid (aa) residue precursor protein with a 28 aa residue signal peptide, a 222 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 155 aa residue intracellular domain. The extracellular region contains four cysteine-rich domains and binds NGF, BDNF, NT-3, and NT-4 approximately equally with low affinity. The cytoplasmic region of the receptor contains a subtype 2 death domain. NGF R expression has been shown to occur widely during development and in the adult. Expression has been detected in both neuronal and non-neuronal cells. NGF R was originally reported to function as a positive regulator of TrkA activity. NGF R has also been shown to signal by itself. Depending on its cellular environment, NGF R has now been shown to regulate cell migration, gene expression and to mediate apoptosis. Recombinant NGF R Fc chimera binds NGF with high affinity and is a potent NGF antagonist. Naturally occurring truncated NGF R containing the extracellular domain and lacking the transmembrane or intracellular domain has been detected in vivo in urine, plasma, and in the amniotic fluid of humans and rats (1-3). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse NGF R/TNFRSF16 by Western Blot. Western blot shows lysates of mouse uterus tissue. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse NGF R/TNFRSF16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1157) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for NGF R/TNFRSF16 at approximately 60-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
NGF R/TNFRSF16 in Mouse Brain. NGF R/TNFRSF16 was detected in perfusion fixed frozen sections of mouse brain (cortex) using 7 µg/mL Goat Anti-Mouse NGF R/TNFRSF16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1157) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections. |
