| 货号 | AF1141-SP |
| 别名 | HAI; HAI1; HAI-1; hepatocyte growth factor activator inhibitor 1; Hepatocyte growth factor activator inhibitor type 1; kunitz-type protease inhibitor 1; MANSC2; serine peptidase inhibitor, Kunitz type 1; serine protease inhibitor, Kunitz type 1; SPINT1 | 全称 | HGF Activator Inhibitor Type 1 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects mouse HAI-1 in direct ELISAs and Western blots. In Western blots, approximately 40% cross-reactivity with recombinant human HAI‑1 is observed and less than 1% cross-reactivity with recombinant mouse HAI-2B is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunoprecipitation: 25 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6692 (Human); 20732 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. PDZ-RhoGEF and LARG are essential for embryonic development and provide a link between thrombin and LPA receptors and Rho activation. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse HAI-1 Glu30-Glu443 (Cys325Ser) Accession # NP_058603 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Hepatocyte growth factor activator inhibitor-1 (HAI-1) encoded by the Spint1 gene is a Kunitz-type serine protease inhibitor, identified as a strong inhibitor of HGF activator (HGFA) and matripase (1). The membrane-anchored HAI-1 consists of two Kunitz domains, a LDL-receptor-like domain, and a C-terminal transmembrane domain (2). Two soluble forms are generated by ectodomain shedding, one with a single Kunitz domain and the other with two Kunitz domains. HAI-1 is not only an inhibitor but also a specific receptor of active HGFA, acting as a reservoir of this enzyme on the cell surface (3). The shedding of HAI-1 and HGFA/HAI-1 complex is enhanced by treatment with phorbol 12-myristate, 13-acetate or IL-1 beta. The regulated shedding is completely inhibited by a synthetic zinc metalloprotease inhibitor (3). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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