Mouse Adiponectin/Acrp30 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse Adiponectin/Acrp30 Affinity Purified Polyclonal Ab (25 UG)

货号： AF1119-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF1119-SP
别名	ACDC; Acrp30; ACRP30ADPN; adipocyte, C1Q and collagen domain containing; adiponectin; adiponectin, C1Q and collagen domain containing; AdipoQ; ADIPQTL1; ApM1; apM-1; APM1APM-1; C1q and collagen domain-containing protein; GBP28; GBP28apM1; Gelatin-binding protein
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects mouse Adiponectin/Acrp30 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant rat Adiponectin is observed and approximately 30% cross-reactivity with recombinant human Adiponectin is observed.
使用方法	Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	9370 (Human); 11450 (Mouse); 246253 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Ultrastructural localization of adiponectin protein in vasculature of normal and atherosclerotic mice. Authors: Mori T, Koyama Y, Maeda N, Nakamura Y, Fujishima Y, Matsuda K, Funahashi T, Shimada S, Shimomura I Sci Rep, 2014;4(0):4895. Species: Mouse Sample Type: Whole Tissue Application: IHC OCT-embedded T-cadherin is essential for adiponectin-mediated revascularization. Authors: Parker-Duffen, Jennifer, Nakamura, Kazuto, Silver, Marcy, Kikuchi, Ryosuke, Tigges, Ulrich, Yoshida, Sumiko, Denzel, Martin S, Ranscht, Barbara, Walsh, Kenneth J Biol Chem, 2013;288(34):24886-97. Species: Mouse Sample Type: tissue Application: WB Distinct fibroblast lineages determine dermal architecture in skin development and repair. Authors: Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F Nature, 2013;504(7479):277-81. Species: Mouse Sample Type: tissue Application: IHC Frozen Vascular smooth muscle cell-derived adiponectin: a paracrine regulator of contractile phenotype. Authors: Ding M, Carrao A, Wagner R, Xie Y, Jin Y, Rzucidlo E, Yu J, Li W, Tellides G, Hwa J, Aprahamian T, Martin K J Mol Cell Cardiol, 2012;52(2):474-84. Species: Mouse Sample Type: Whole Cells Application: ICC Molecular, biochemical and functional characterizations of C1q/TNF family members: adipose-tissue-selective expression patterns, regulation by PPAR-gamma agonist, cysteine-mediated oligomerizations, combinatorial associations and metabolic functions. Authors: Wong GW, Krawczyk SA, Kitidis-Mitrokostas C, Revett T, Gimeno R, Lodish HF Biochem. J., 2008;416(2):161-77. Species: Human Sample Type: recombinant protein Application: WB Adiponectin modulates inflammatory reactions via calreticulin receptor-dependent clearance of early apoptotic bodies. Authors: Takemura Y, Ouchi N, Shibata R, Aprahamian T, Kirber MT, Summer RS, Kihara S, Walsh K J. Clin. Invest., 2007;117(2):375-86. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse Adiponectin/Acrp30 (R&D Systems, Catalog # 5095-AC) Glu18-Asn247 Accession # Q60994
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Adiponectin, also known as Acrp30, is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity, and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. In this context, adiponectin is an anti-inflammatory agent, but it exerts pro-inflammatory effects in nonmetabolic disorders such as rheumatoid arthritis and inflammatory bowel disease (1-3). Adiponectin interacts with the receptors AdipoR1 and AdipoR2, calreticulin, and Cadherin-13/T-Cadherin, as well as with several growth factors (4-7). Mature mouse adiponectin consists of a 66 amino acid (aa) N-terminal collagenous region and a 137 aa C-terminal C1q-like globular domain which can be cleaved by a leukocyte-derived elastase (8-10). Mature mouse adiponectin shares 83% and 91% amino acid (aa) sequence identity with human and rat adiponectin, respectively. Adiponectin associates into trimers that may assemble into medium molecular weight (MMW) hexamers and then into >300 kDa high molecular weight (HMW) oligomers (11-13). The glycosylation of four hydroxylated lysine residues in the collagenous domain is required for the intracellular formation of HMW complexes (14). The various multimeric forms of adiponectin exhibit distinct tissue specific and gender specific profiles and activities (13, 15).
运输条件	Blue Ice
存放说明	4℃
参考文献	Lara-Castro, C. et al. (2007) Curr. Opin. Lipidol. 18:263. Tilg, H. and A.R. Moschen (2006) Nat. Rev. Immunol. 6:772. Fantuzzi, G. (2008) J. Allergy Clin. Immunol. 121:326. Yamauchi, T. et al. (2007) Nat. Med. 13:332. Takemura, Y. et al. (2007) J. Clin. Invest. 117:375. Hug, C. et al. (2004) Proc. Natl. Acad. Sci. USA 101:10308. Wang, Y. et al. (2005) J. Biol. Chem. 280:18341. Scherer, P.E. et al. (1995) J. Biol. Chem. 270:26746. Hu, E. et al. (1996) J. Biol. Chem. 271:10697. Waki, H. et al. (2005) Endocrinology 146:790. Waki, H. et al. (2003) J. Biol. Chem. 278:40352. Tsao, T.S. et al. (2003) J. Biol. Chem. 278:50810. Wang, Y. et al. (2008) Biochem. J. 409:623. Wang, H. et al. (2006) J. Biol. Chem. 281:16391. Pajvani, U.B. et al. (2003) J. Biol. Chem. 278:9073.

参考图片

Adiponectin/Acrp30 in ST-2 mouse bone marrow-derived stromal cell line differentiated to adipocytes. Adiponectin/Acrp30 was detected in immersion fixed ST-2 mouse bone marrow-derived stromal cell line differentiated to adipocytes using Goat Anti-Mouse Adiponectin/Acrp30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1119) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Adiponectin/Acrp30 in human mesenchymal stem cells differentiated to adipocytes. Adiponectin/Acrp30 was detected in immersion fixed human mesenchymal stem cells differentiated to adipocytes using Goat Anti-Mouse Adiponectin/Acrp30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1119) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.