| 货号 | AF1085-SP |
| 别名 | artemin; ARTN; Enovin; EVN; EVNneurotrophic factor; NBN; Neublastin |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse Artemin in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) GDNF, recombinant rat GDNF, rhNeurturin, and rhTGF-beta 1 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize Artemin-induced proliferation in the SH‑SY5Y human neuroblastoma cell line. The Neutralization Dose (ND50) is typically 0.1-0.3 µg/mL in the presence of 15 ng/mL Recombinant Mouse Artemin. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 9048 (Human); 11876 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Glial-cell-derived neuroregulators control type 3 innate lymphoid cells and gut defence | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse Artemin Ala112-Gly224 Accession # Q9Z0L2 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Artemin is a member of the Glia Cell-Derived Neurotrophic factor (GDNF) family ligands, which include GDNF, Persephin, Artemin, and Neurturin. GDNF family ligands are distant members of the Transforming Growth Factor beta (TGF‑ beta ) superfamily (1‑4). Similar to other TGF‑ beta family proteins, Artemin is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. The carboxy-terminal domain of Artemin contains the characteristic seven conserved cysteine residues necessary for the formation of the cysteine-knot and the single interchain disulfide bond. Biologically active Artemin is a disulfide‑linked homodimer of the carboxy-terminal 113 amino acid residues. Mature mouse Artemin shares 88.5% amino acid sequence similarity with human Artemin. Mature Artemin also shares approximately 40% amino acid sequence identity with the other three members of the GDNF family ligands (5). Bioactivities of all GDNF family ligands are mediated through a receptor complex composed of a high affinity ligand binding component (GFR alpha 1‑GFR alpha 4) and a common signaling component, cRET (receptor tyrosine kinase) (5‑8). Artemin prefers to bind to GFR alpha 3 and activites the GFR alpha 3‑RET. However, in the presence of RET, it can bind to GFR alpha 1 as well (4, 5, 9). Artemin has been shown to promote the survival and growth of various peripheral and central neurons, including sympathetic and dopaminergic neurons. It may also play an important role in the development of sympathetic neurons and several organs (5, 10, 11). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Cell Proliferation Induced by Artemin and Neutralization by Mouse Artemin Antibody. Recombinant Mouse Artemin (Catalog # 1085-AR) stimulates proliferation in the SH‑SY5Y human neuroblastoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Artemin (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Artemin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1085). The ND50 is typically 0.1-0.3 µg/mL. |
