Mouse PDGF R beta Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse PDGF R beta Affinity Purified Polyclonal Ab (25 UG)

货号： AF1042-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF1042-SP
别名	beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; CD140b antigen; CD140b; EC 2.7.10; EC 2.7.10.1; JTK12; PDGFR; PDGFR1; PDGFRB; PDGF-R-beta; platelet-derived growth factor receptor, beta polypeptide
全称	Platelet-derived Growth Factor Receptor beta
反应种属	Mouse
应用	Western Blot(0.25 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects mouse PDGF R beta in direct ELISAs and Western blots. In direct ELISAs, this antibody shows approximately 35% cross-reactivity with recombinant human PDGF R beta.
使用方法	Western Blot: 0.25 µg/mL Immunohistochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5159 (Human); 18596 (Mouse); 24629 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Microglia protect against brain injury and their selective elimination dysregulates neuronal network activity after stroke Nat Commun, 2016;7(0):11499. Species: Mouse Sample Type: Whole Tissue Application: IHC Not Specified Multiple mouse models of primary lymphedema exhibit distinct defects in lymphovenous valve development. Authors: Geng X, Cha B, Mahamud M, Lim K, Silasi-Mansat R, Uddin M, Miura N, Xia L, Simon A, Engel J, Chen H, Lupu F, Srinivasan R Dev Biol, 2016;409(1):218-33. Species: Mouse Sample Type: Whole Tissue Application: IHC-OCT embedded Enhanced sphingosine-1-phosphate receptor 2 expression underlies female CNS autoimmunity susceptibility. Authors: Cruz-Orengo L, Daniels B, Dorsey D, Basak S, Grajales-Reyes J, McCandless E, Piccio L, Schmidt R, Cross A, Crosby S, Klein R J Clin Invest, 2014;124(6):2571-84. Species: Mouse Sample Type: Whole Tissue Application: IHC - Not specified Pericyte loss influences Alzheimer-like neurodegeneration in mice. Authors: Sagare A, Bell R, Zhao Z, Ma Q, Winkler E, Ramanathan A, Zlokovic B Nat Commun, 2013;4(0):2932. Species: Mouse Sample Type: tissue Application: IHC Frozen Survival effect of PDGF-CC rescues neurons from apoptosis in both brain and retina by regulating GSK3beta phosphorylation. Authors: Tang Z, Arjunan P, Lee C, Li Y, Kumar A, Hou X, Wang B, Wardega P, Zhang F, Dong L, Zhang Y, Zhang SZ, Ding H, Fariss RN, Becker KG, Lennartsson J, Nagai N, Cao Y, Li X J. Exp. Med., 2010;207(4):867-80. Species: Mouse Sample Type: In Vivo Application: In vivo Characterization of neuroprogenitor cells expressing the PDGF beta-receptor within the subventricular zone of postnatal mice. Authors: Ishii Y, Matsumoto Y, Watanabe R, Elmi M, Fujimori T, Nissen J, Cao Y, Nabeshima Y, Sasahara M, Funa K Mol. Cell. Neurosci., 2007;37(3):507-18. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Tumor-driven paracrine platelet-derived growth factor receptor alpha signaling is a key determinant of stromal cell recruitment in a model of human lung carcinoma. Authors: Tejada ML, Yu L, Dong J, Jung K, Meng G, Peale FV, Frantz GD, Hall L, Liang X, Gerber HP, Ferrara N Clin. Cancer Res., 2006;12(9):2676-88. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse PDGF R beta Leu32-Lys530 Accession # P05622
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	The platelet-derived growth factor (PDGF) family consists of proteins derived from four genes (PDGF-A, -B, -C, and -D) that form disulfide-linked homodimers (PDGF-AA, -BB, -CC, and -DD) and a heterodimer (PDGF-AB) (1, 2). These proteins regulate diverse cellular functions by binding to and inducing the homo- or hetero-dimerization of two receptors (PDGF R alpha and R beta ). Whereas alpha / alpha homo-dimerization is induced by PDGF-AA, -BB, -CC, and -AB, alpha / beta hetero-dimerization is induced by PDGF-AB, -BB, -CC, and -DD, and beta / beta homo-dimerization is induced only by PDGF-BB, and -DD (1 - 4). Both PDGF R alpha and R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3-ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. Ligand-induced receptor dimerization results in autophosphorylation in trans resulting in the activation of several intracellular signaling pathways that can lead to cell proliferation, cell survival, cytoskeletal rearrangement, and cell migration. Many cell types, including fibroblasts and smooth muscle cells, express both the alpha and beta receptors. Others have only the alpha receptors (oligodendrocyte progenitor cells, mesothelial cells, liver sinusoidal endothelial cells, astrocytes, platelets and megakaryocytes) or only the beta receptors (myoblasts, capillary endothelial cells, pericytes, T cells, myeloid hematopoietic cells and macrophages). A soluble PDGF R alpha has been detected in normal human plasma and serum as well as in the conditioned medium of the human osteosarcoma cell line MG-63 (5). Both the recombinant mouse and human soluble PDGF R alpha bind PDGF with high affinity and are potent PDGF antagonists.
运输条件	Blue Ice
存放说明	4℃
参考文献	Betshotz, C. et al. (2001) BioEssays 23:494. Ostman, A. and A.H. Heldin (2001) Advances in Cancer Research 80:1. Gilbertson, D. et al. (2001) J. Biol. Chem. 276:27406. LaRochells, W.J. et al. (2001) Nature Cell Biol. 3:517. Tiesman, J. and C.E. Hart (1993) J. Biol. Chem. 5:9621.

参考图片

Detection of Rat and Mouse PDGF R beta by Western Blot. Western blot shows lysates of rat spinal cord tissue, rat brain tissue, and mouse brain tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1042) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PDGF R beta at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

PDGF R beta in Mouse Embryonic Spinal Cord. PDGF R beta was detected in immersion fixed frozen sections of mouse embryo spinal cord using 5 µg/mL Goat Anti-Mouse PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1042) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.