Mouse Cathepsin C/DPPI Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse Cathepsin C/DPPI Affinity Purified Polyclonal Ab (25 UG)

货号： AF1034-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF1034-SP
别名	cathepsin CEC 3.4.14.1; Cathepsin J; CPPIHMS; CTSC; dipeptidyl peptidase 1; Dipeptidyl peptidase I; Dipeptidyl transferase; dipeptidyl-peptidase I; DPP1; DPPI; DPP-I; JP; JPD; PALS; PLS
反应种属	Mouse
应用	Western Blot(0.25 µg/mL) Simple Western(2.5 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects mouse Cathepsin C/DPPI in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross‑reactivity with recombinant human (rh) Cathepsin C is observed and less than 1% cross-reactivity with recombinant mouse (rm) Cathepsin A, rmCathepsin B, rmCathepsin D, rmCathepsin H, and rmCathepsin X/Z/P is observed.
使用方法	Western Blot: 0.25 µg/mL Simple Western: 2.5 µg/mL Immunohistochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1075 (Human); 13032 (Mouse); 25423 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Lysosomal protein turnover contributes to the acquisition of TGFbeta-1 induced invasive properties of mammary cancer cells. Authors: Kern U, Wischnewski V, Biniossek M, Schilling O, Reinheckel T Mol Cancer, 2015;14(0):39. Species: Mouse Sample Type: Cell Lysates Application: WB Deficiency for the cysteine protease cathepsin L impairs Myc-induced tumorigenesis in a mouse model of pancreatic neuroendocrine cancer. Authors: Brindle N, Joyce J, Rostker F, Lawlor E, Swigart-Brown L, Evan G, Hanahan D, Shchors K PLoS ONE, 2015;10(4):e0120348. Species: Mouse Sample Type: Whole Tissue Application: IHC-OCT embedded Up-regulation of microglial cathepsin C expression and activity in lipopolysaccharide-induced neuroinflammation. Authors: Fan K, Wu X, Fan B, Li N, Lin Y, Yao Y, Ma J J Neuroinflammation, 2012;9(1):96. Species: Human Sample Type: Whole Tissue Application: IHC Macrophages and cathepsin proteases blunt chemotherapeutic response in breast cancer. Authors: Shree T, Olson OC, Elie BT Genes Dev., 2011;25(23):2465-79. Species: Mouse Sample Type: Tissue Homogenates Application: WB A role for serglycin proteoglycan in mast cell apoptosis induced by a secretory granule-mediated pathway. Authors: Melo FR, Waern I, Ronnberg E, Abrink M, Lee DM, Schlenner SM, Feyerabend TB, Rodewald HR, Turk B, Wernersson S, Pejler G J. Biol. Chem., 2011;286(7):5423-33. Species: Mouse Sample Type: Cell Lysates Application: WB Mice deficient in LMAN1 exhibit FV and FVIII deficiencies and liver accumulation of {alpha}1-antitrypsin. Authors: Zhang B, Zheng C, Zhu M, Tao J, Vasievich MP, Baines A, Kim J, Schekman R, Kaufman RJ, Ginsburg D Blood, 2011;118(12):3384-91. Species: Mouse Sample Type: Whole Cells Application: ICC Gene targeting of the cysteine peptidase cathepsin H impairs lung surfactant in mice. Authors: Buhling F, Kouadio M, Chwieralski CE, Kern U, Hohlfeld JM, Klemm N, Friedrichs N, Roth W, Deussing JM, Peters C, Reinheckel T PLoS ONE, 2011;6(10):e26247. Species: Mouse Sample Type: Tissue Homogenates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin C/DPPI Asp25-Leu462 Accession # P97821
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Cathepsin C is a cysteine protease of the papain family (1). Cathepsin C sequentially removes dipeptides from the free N-termini of proteins and peptides. It has broad specificity except that it does not cleave a basic amino acid (Arg or Lys) in the N-terminal position or Pro on either side of the scissle bond. It requires halide ions for activity. The pro form contains a pro peptide and a catalytic region, which can be further processed into heavy/ alpha and light/ beta chains that are linked by a disulfide bond. It is broadly distributed. Cathepsin C plays a role in the lysosomal degradation. It also functions as a key enzyme in the activation of granule serine proteases in cytotoxic T lymphocytes and natural killer cells (granzymes A and B), mast cells (tryptase and chymase), and neutrophils (Cathepsin G and elastase) by removing their N-terminal activation dipeptides (2).
运输条件	Blue Ice
存放说明	4℃
参考文献	Turk, B. et al. (2004) in Handbook of Proteolytic Enzymes. Barrett,et al. eds. p. 1192, Academic Press, San Diego. Dahl, S.W. et al. (2001) Biochemistry 40:1671.

参考图片

Detection of Mouse Cathepsin C/DPPI by Western Blot. Western blot shows lysates of mouse liver tissue and mouse spleen tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Cathepsin C/DPPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1034) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Cathepsin C/DPPI at approximately 20-25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Cathepsin C/DPPI in Mouse Thymus. Cathepsin C/DPPI was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse Cathepsin C/
DPPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1034) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to thymocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Mouse Cathepsin C/DPPI by Simple Western^TM. Simple Western lane view shows lysates of mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for Cathepsin C/DPPI at approximately 35 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse Cathepsin C/DPPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1034) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.