Temporal expression of growth factors triggered by epiregulin regulates inflammation development.
Authors: Harada M, Kamimura D, Arima Y, Kohsaka H, Nakatsuji Y, Nishida M, Atsumi T, Meng J, Bando H, Singh R, Sabharwal L, Jiang J, Kumai N, Miyasaka N, Sakoda S, Yamauchi-Takihara K, Ogura H, Hirano T, Murakami M
J Immunol, 2015;194(3):1039-46.
Species: Mouse
Sample Type: In Vivo
Application: In Vivo
ZEB1 sensitizes lung adenocarcinoma to metastasis suppression by PI3K antagonism.
Authors: Yang Y, Ahn Y, Chen Y, Tan X, Guo L, Gibbons D, Ungewiss C, Peng D, Liu X, Lin S, Thilaganathan N, Wistuba I, Rodriguez-Canales J, McLendon G, Creighton C, Kurie J
J Clin Invest, 2014;124(6):2696-708.
Species: Mouse
Sample Type: Total protein
Application: WB
Betacellulin overexpression in the mouse ovary leads to MAPK3/MAPK1 hyperactivation and reduces litter size by impairing fertilization.
Authors: Gratao AA, Dahlhoff M, Sinowatz F, Wolf E, Schneider MR
Biol. Reprod., 2007;78(1):43-52.
Species: Mouse
Sample Type: Tissue Homogenates
Application: WB
Selective roles for tumor necrosis factor alpha-converting enzyme/ADAM17 in the shedding of the epidermal growth factor receptor ligand family: the juxtamembrane stalk determines cleavage efficiency.
Authors: Hinkle CL, Sunnarborg SW, Loiselle D, Parker CE, Stevenson M, Russell WE, Lee DC
J. Biol. Chem., 2004;279(23):24179-88.
Species: Mouse
Sample Type: Cell Lysates
Application: ELISA Development

Betacellulin (BTC) is a member of the EGF family of cytokines which includes EGF, TGF-alpha, amphiregulin, HB-EGF, epiregulin, tomoregulin and the neuregulins. All EGF famiy members are synthesized as type I transmembrane precursor proteins containing one or more EGF-like domains in their extracellular region (1). BTC, a heparin-binding protein, was originally isolated from the conditioned media of mouse pancreatic beta tumor cells as a 32 kDa glycoprotien (2). The mouse BTC cDNA encodes a 177 amino acid (aa) residue precursor with a 31 aa signal peptide, an 87 aa residue extracellular region containing one EGF-like domain, a 21 aa transmembrane domain and a 38 aa cytoplasmic domain. Soluble BTC is released from the transmembrane precursor by proteolytic processing (3). Mouse BTC shares 93% and 79% aa sequence identity with rat and human BTC, respectively (1). The mouse BTC gene is tightly linked to that of amphiregulin on mouse chromosome 5 (4). BTC is expressed in most tissues including kidney, uterus, liver and pancreas. It is also present in body fluids including serum, milk and colostrum (5). BTC promotes pancreatic beta-cell growth and differentiation (6) and is a potent mitogen for retinal pigment epithelial cells, vascular smooth muscle cells and fibroblasts (1). The effects of BTC is mediated by binding to ErbB1 and ErbB4 homodimers as well as ErbB heterodimers (1).

1. Dunbar, A.J. and C. Goddard (2000) Int. J. Biochem. Cell Biol. 32:805.
2. Shing, Y. et al. (1993) Science 259:1604.
3. Tada, H. et al. (1999) J. Cell Biochem. 72:423.
4. Pathak, B.G. et al. (1995) Genomics 28:116.
5. Bastian, S.E. et al. (2001) J. Endocrinol. 168:203.
6. Li, L. et al. (2001) Endocrinology 142:5379.