Mouse B7-H1 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse B7-H1 Affinity Purified Polyclonal Ab (25 UG)

货号： AF1019-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF1019-SP
别名	B7-H; B7-H1; B7H1PDCD1L1; CD274 antigenMGC142294; CD274 molecule; CD274; PDCD1L1; PDCD1LG1; PDCD1LG1MGC142296; PD-L1; PD-L1B7 homolog 1; PDL1PDCD1 ligand 1; programmed cell death 1 ligand 1; Programmed death ligand 1
全称	B7 Homolog 1
反应种属	Mouse
应用	Western Blot(0.5 µg/mL) Flow Cytometry(0.25 µg/10⁶cells) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects mouse B7‑H1/PD‑L1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human B7-H1 is observed.
使用方法	Western Blot: 0.5 µg/mL Flow Cytometry: 0.25 µg/10⁶cells Immunohistochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	29126 (Human); 60533 (Mouse); 102145573 (Cynomolgus Monkey)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Cell autonomous or systemic EGFR blockade alters the immune-environment in squamous cell carcinomas Int J Cancer, 2016;0(0):. Species: Mouse Sample Type: Whole Tissue Application: IHC - Paraffin embedded PD-1/PD-L1 blockade enhances T cell activity and antitumor efficacy of imatinib in gastrointestinal stromal tumors Clin Cancer Res, 2016;0(0):. Species: Mouse Sample Type: Whole Tissue Application: IHC - Paraffin embedded Endogenous retinoids in the pathogenesis of alopecia areata. Authors: Duncan F, Silva K, Johnson C, King B, Szatkiewicz J, Kamdar S, Ong D, Napoli J, Wang J, King L, Whiting D, McElwee K, Sundberg J, Everts H J Invest Dermatol, 2013;133(2):334-43. Species: Mouse Sample Type: tissue Application: IHC - Not specified Anti-programmed cell death 1 antibody reduces CD4+PD-1+ T cells and relieves the lupus-like nephritis of NZB/W F1 mice. Authors: Kasagi S, Kawano S, Okazaki T, Honjo T, Morinobu A, Hatachi S, Shimatani K, Tanaka Y, Minato N, Kumagai S J. Immunol., 2010;184(5):2337-47. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Altered availability of PD-1/PD ligands is associated with the failure to control autoimmunity in NOD mice. Authors: Yadav D, Hill N, Yagita H, Azuma M, Sarvetnick N Cell. Immunol., 2009;258(2):161-71. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Establishment of NOD-Pdcd1-/- mice as an efficient animal model of type I diabetes. Authors: Wang J, Yoshida T, Nakaki F, Hiai H, Okazaki T, Honjo T Proc. Natl. Acad. Sci. U.S.A., 2005;102(33):11823-8. Species: Mouse Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse B7‑H1/PD‑L1 Phe19-Thr238 Accession # Q9EP73
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Mouse B7 homolog 1(B7-H1), also called programmed death ligand 1 (PD-L1) and programmed cell death 1 ligand 1 (PDCD1L1), is a member of the B7 family of proteins that provide signals for regulating T-cell activation and tolerance (1‑4). Other family members include B7-1, B7-2, B7-H2, B7-H3 and PD-L2. B7 proteins are immunoglobulin (Ig) superfamily members with extracellular Ig-V-like and Ig-C-like domains and a short cytoplasmic region. Among the family members, they share from 20‑40% amino acid (aa) sequence identity. The cloned mouse B7-H1/PD-L1 cDNA encodes a 290 aa type I membrane precursor protein with a putative 18 aa signal peptide, a 220 aa extracellular region containing one V-like and one C-like Ig domain, a 22 aa transmembrane region, and a 30 aa cytoplasmic domain. Mouse and human B7-H1/PD-L1 share approximately 70% aa sequence identity. B7-H1/PD-L1 is one of two ligands for programmed death-1 (PD-1), a member of the CD28 family of immunoreceptors. The other identified ligand is PD-L2. Mouse B7-H1/PD-L1 and PD-L2 share approximately 34% aa sequence identity and have similar functions. B7-H1/PD-L1 is constitutively expressed in various lymphoid and non-lymphoid organs including placenta, heart, pancreas, lung, liver, and endothelium (1‑4). The expression of B7-H1/PD-L1 is detected on B cells, T cells, monocytes, dendritic cells and thymic epithelial cells. IFN-gamma treatment induces B7-H1/PD-L1 expression in monocytes, dendritic cells, and endothelial cells. B7-H1/PD-L1 expression is also upregulated in a variety of tumor cell lines. On previously activated T cells, B7-H1/PD-L1 interaction with PD-1 inhibits TCR-mediated proliferation and cytokine production, suggesting an inhibitory role in regulating immune responses. In contrast, a costimulatory function for the PD-1 ligands on resting T cells has also been reported (1‑4).
运输条件	Blue Ice
存放说明	4℃
参考文献	Tamura, H. et al. (2001) Blood 97:1809. Freeman, G. et al. (2000) J. Exp. Med. 192:1027. Sharpe, A.H. and G. J. Freeman (2002) Nat. Rev. Immunol. 2:116. Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.

参考图片

Detection of Mouse B7‑H1/PD‑L1 by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with
10 μg/mL LPS for 4 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse B7‑H1/PD‑L1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1019) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for B7‑H1/PD‑L1 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of B7‑H1/PD‑L1 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line either treated with LPS overnight (filled histogram) or untreated (open histogram) was stained with Goat Anti-Mouse B7‑H1/PD‑L1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1019), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.

Detection of B7‑H1/PD‑L1 in HEK293 Human Cell Line Transfected with Mouse B7-H1/PD-L1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse B7-H1/PD-L1 or (B) irrelevant transfectants and eGFP was stained with Goat Anti-Mouse B7‑H1/PD‑L1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1019) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). Quadrant markers were set based on control antibody staining (Catalog # AB-108-C). View our protocol for Staining Membrane-associated Proteins.