Mouse Cathepsin H Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse Cathepsin H Affinity Purified Polyclonal Ab (25 UG)

货号： AF1013-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF1013-SP
别名	ACC-4; ACC-5; aleurain; cathepsin B3; cathepsin BA; cathepsin H; CPSB; CTSH; DKFZp686B24257; EC 3.4.22; EC 3.4.22.16; MGC1519; minichain; N-benzoylarginine-beta-naphthylamide hydrolase
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunoprecipitation(25 µg/mL)
目标/特异性	Detects mouse Cathepsin H in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant human (rh) Cathepsin H is observed and less than 2% cross-reactivity with recombinant mouse (rm) Cathepsin B, rmCathepsin C, rmCathepsin Z, rmCathepsin L, and rhCathepsin O is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunoprecipitation: 25 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1512 (Human); 13036 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Lysosomal protein turnover contributes to the acquisition of TGFbeta-1 induced invasive properties of mammary cancer cells. Authors: Kern U, Wischnewski V, Biniossek M, Schilling O, Reinheckel T Mol Cancer, 2015;14(0):39. Species: Mouse Sample Type: Cell Lysates Application: WB Macrophages and cathepsin proteases blunt chemotherapeutic response in breast cancer. Authors: Shree T, Olson OC, Elie BT Genes Dev., 2011;25(23):2465-79. Species: Mouse Sample Type: Tissue Homogenates Application: WB Gene targeting of the cysteine peptidase cathepsin H impairs lung surfactant in mice. Authors: Buhling F, Kouadio M, Chwieralski CE, Kern U, Hohlfeld JM, Klemm N, Friedrichs N, Roth W, Deussing JM, Peters C, Reinheckel T PLoS ONE, 2011;6(10):e26247. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin H Glu22-Val333 Accession # Q3UCD6
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Cathepsin H is a lysosomal cysteine protease of the papain family (1). It is synthesized as a precursor protein, consisting of a signal peptide (residues 1‑20), a propeptide (residues 21‑95), a mini chain (residues 96‑103), a heavy chain (residues 114‑290) and a light chain (residues 291‑333) (2, 3). A truncated form with a 12 amino acid deletion in the signal peptide region is secreted (4). Cathepsin H is the only known mono-aminopeptidase in the papain family (5). Cathepsin H expression is significantly increased in disease states such as in prostate tumors, sera of asthmatic patients, and mucosa of colorectal cancer patients (4, 6, 7).
运输条件	Blue Ice
存放说明	4℃
参考文献	Kirschke, H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 1089, Academic Press, San Diego. Lafuse, W.P. et al. (1995) J. Leukoc. Biol. 57:663. Soderstrom, M. et al. (1999) Biochim. Biophys. Acta 1446:35. Waghray, A. et al. (2002) J. Biol. Chem. 277:11533. Guncar, G. et al. (1998) Structure 6:51. Cimerman, N. et al. (2001) Clin. Chim. Acta 310:113. del Re, E.C. et al. (2000) Br. J. Cancer. 82:1317.

参考图片

Cathepsin H in Mouse Ovary.

Cathepsin H was detected in perfusion fixed frozen sections of mouse ovary using 1.7 µg/mL Goat Anti-Mouse Cathepsin H Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1013) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.