| 货号 | AF1004-SP |
| 别名 | DER4; Fit-1; IL-1 R4; IL-1RL1; IL-33R; Ly84; ST2L; ST2V; suppression of tumorigenicity 2; T1 | 全称 | Interleukin 33 Receptor |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects mouse ST2/IL-33 R in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human ST2/IL-33 R is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize IL‑33-induced proliferation in the D10.G4.1 mouse helper T cell line. Schmitz, J. et al. (2005) Immunity 23:479. The Neutralization Dose (ND50) is typically 1-6 μg/mL in the presence of 1 ng/mL Recombinant Human IL‑33 and Hamster Anti-Mouse CD3 epsilon Monoclonal Antibody. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 9173 (Human); 17082 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. IL-33 is produced by mast cells and regulates IgE-dependent inflammation. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse ST2/IL-33 R Ser27-Arg332 Accession # P14719 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | ST2, also known as IL-1 R4 and T1, is an Interleukin-1 receptor family glycoprotein that contributes to Th2 immune responses. Mouse ST2 consists of a 306 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 23 aa transmembrane segment, and a 212 aa cytoplasmic domain with an intracellular TIR domain. Alternate splicing of the 120 kDa mouse ST2 generates a soluble 60 kDa isoform that lacks the transmembrane and cytoplasmic regions. Within the ECD, mouse ST2 shares 68% and 81% aa sequence identity with human and rat ST2, respectively. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes. It binds IL-33, a cytokine that is upregulated by inflammation or mechanical strain in smooth muscle cells, airway epithelia, keratinocytes, and cardiac fibroblasts. IL-33 binding induces the association of ST2 with IL-1R AcP, a shared signaling subunit that also associates with IL-1 RI and IL‑1 R rp2. In macrophages, ST2 interferes with signaling from IL-1 RI and TLR4 by sequestering the adaptor proteins MyD88 and Mal. In addition to its role in promoting mast cell and Th2 dependent inflammation, ST2 activation enhances antigen induced hypernociception and protects from atherosclerosis and cardiac hypertrophy. The soluble ST2 isoform is released by activated Th2 cells and strained cardiac myocytes and is elevated in the serum in allergic asthma. Soluble ST2 functions as a decoy receptor that blocks IL-33’s ability to signal through transmembrane ST2. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Cell Proliferation Induced by IL‑33 and Neutralization by Mouse ST2/IL-33 R Antibody. Recombinant Human IL‑33 (Catalog # 3625-IL) induces proliferation in the D10.G4.1 mouse helper T cell line in the presence of Hamster Anti-Mouse CD3 epsilon Monoclonal Antibody (Catalog # MAB484) in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Under these conditions, proliferation elicited by IL‑33 is neutralized (green line) by increasing concentrations of Goat Anti-Mouse ST2/IL-33 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1004). The ND50 is typically 1-6 μg/mL. |
