| 货号 | AF1001-SP |
| 别名 | frizzled (Drosophila) homolog 3; frizzled homolog 3 (Drosophila); frizzled-3; Fz-3; FZD3; hFz3 |
| 反应种属 | Mouse |
| 应用 | Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse Frizzled-3 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) Frizzled-1, rmFrizzled-4, rmFrizzled-6, rmFrizzled-7, and rmFrizzled-8 is observed. |
| 使用方法 | Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7976 (Human); 14365 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Wnt signaling in the pathogenesis of human HIV-associated pain syndromes. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-3 His23-Val157 Accession # Q61086 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Frizzled-3 is an integral membrane protein that belongs to the Frizzled family of G-protein coupled receptors. Frizzleds function as receptors for Wnt proteins. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Frizzled‑3 in Mouse Embryo. Frizzled‑3 was detected in immersion fixed frozen sections of mouse embryo (E11) using Mouse Frizzled‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1001) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
