Viral MIP-I Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Viral MIP-I Affinity Purified Polyclonal Ab (25 UG)

货号： AF799-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF799-SP
别名	MIPI; MIP-I
全称	Macrophage Inflammatory Protein I
应用	Western Blot(0.1 µg/mL)
目标/特异性	Detects viral MIP‑I in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 2% cross‑reactivity with recombinant viral MIP-II is observed.
使用方法	Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize MIP‑I-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR8. The Neutralization Dose (ND₅₀) is typically 0.8-4.0 µg/mL in the presence of 0.02 µg/mL Recombinant Viral MIP‑I.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human herpes virus-8 MIP-I Ala25-Ala95 Accession # NP_572066
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Viral
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Human herpesvirus-8 (HHV-8)/Kaposi’s sarcoma-associated herpesvirus (KSHV) encodes a variety of immunomodulatory proteins which were apparently pirated from cellular genes by the virus. Three chemokine-like proteins, vMIP-I, vMIP-II and vMIP-III have been found within the HHV-8 genome. Viral MIP-II cDNA encodes a 94 amino acid (aa) residue precursor protein with a 23 aa residue signal peptide that is cleaved to yield a 71 aa residue mature protein. Among human chemokines, vMIP-II is most closely related to MIP-1 alpha, sharing approximately 41% amino acid sequence identity. At the amino acid sequence level, vMIP-I and vMIP-II also share 48% identity.
运输条件	Blue Ice
存放说明	4℃
参考文献	CC Chemokines and Receptors Chemokines and Receptors in Angiogenesis Infectious Diseases Viral Cytokines

参考图片

Chemotaxis Induced by MIP‑I and Neutralization by Viral MIP‑I Antibody.
Recombinant Viral MIP‑I (Catalog # 600‑VA) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR8 a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinan Viral MIP‑I (0.02 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Viral MIP-I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF799). The ND₅₀ is typically 0.8‑4.0 µg/mL.