| 货号 | AF697-SP |
| 别名 | MCV-type II Chemokine-like Protein; MCVtype II | 全称 | Molluscum Contagiosum Virus Type II |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects viral MCV-type II Chemokine-like Protein in direct ELISAs and Western blots. In direct ELISAs, no cross‑reactivity with ________________ is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize MCV‑type II Chemokine‑like Protein inhibition of CCL1/I‑309-dependent chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR8. The Neutralization Dose (ND50) is typically 5-20 µg/mL in the presence of 0.2 µg/mL Recombinant Viral MCV‑type II Chemokine‑like Protein and 20 #ng/mL Recombinant Human CCL1/I‑309. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant viral MCV-type II Chemokine-like Protein |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Viral |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Molluscum contagiosum virus (MCV) is a human cutaneous poxvirus that has been shown to encode homologs of cellular immunomodulatory proteins. MC148R2 is a CC chemokine-like protein encoded by an MCV type II gene. MC148R2 is a 104 amino acid (aa) protein containing a 24 aa signal peptide that is cleaved to produce an 80 aa mature protein. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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MCV‑type II Chemokine‑like Protein Inhibition of CCL1/I‑309-dependent Chemotaxis and Neutralization by Viral MCV‑type II Chemokine‑like Protein Antibody. Recombinant Viral MCV‑type II Chemokine‑like Protein (Catalog # 697-M3) inhibits Recombinant Human CCL1/I‑309 (Catalog # 272-I) induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR8 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Inhibition of Recombinant Human CCL1/I‑309 (20 ng/mL) activity elicited by Recombinant Viral MCV‑type II Chemokine‑like Protein (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Viral MCV-type II Chemokine-like Protein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF697). The ND50 is typically 5-20 µg/mL. |
