Viral CCI Affinity Purified Polyclonal Ab (25 UG)
货号：
AF696-SP 基本售价：
1378.1 元 规格：
-
产品信息
概述| 货号 | AF696-SP |
| 别名 | CCI |
全称 | CC Chemokine Inhibitor | | 应用 | Western Blot(0.1 µg/mL)
|
| 目标/特异性 | Detects viral CCI in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.1 µg/mL
Neutralization: Measured by its ability to neutralize CCI inhibition of CCL2/JE/MCP‑1-dependent chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 3-15 µg/mL in the presence of 0.4 µg/mL Recombinant Viral CCI Fc Chimera and 20 ng/mL Recombinant Mouse CCL2/JE/MCP‑1.
|
| 来源 | Polyclonal Goat IgG |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant viral CCI |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Viral |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The family of T1/35 kDa proteins are secreted, soluble proteins encoded by the 35K virulence gene of many poxviruses. These proteins bind CC chemokines with high affinity and are now termed viral chemokine inhibitors (vCCI). Vaccinia virus (Strain Lister) vCCI shows greater than 90% amino acid sequence identity with vCCI from other orthopoxviruses and approximately 40% amino acid sequence identity with the leporipoxvirus T-1 proteins. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - CC Chemokines and Receptors
- Infectious Diseases
|
参考图片CCI Inhibition of CCL2/JE-dependent Chemotaxis and Neutralization by Viral CCI Antibody. Recombinant Viral CCI Fc Chimera (Catalog # 696‑CC) inhibits Recombinant Mouse CCL2/JE (Catalog # 479‑JE) induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Inhibition of Recombinant Mouse CCL2/JE (20 ng/mL) activity elicited by Recombinant Viral CCI Fc Chimera (0.4 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Viral CCI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF696). The ND 50 is typically 3-15 µg/mL. |
