| 货号 | AF117-SP |
| 别名 | CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF | 全称 | Interleukin 10 |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects viral HCMV IL-10 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse IL‑10, recombinant canine IL‑10, recombinant feline IL‑10, recombinant cotton rat IL‑10, recombinant rat IL‑10, recombinant porcine IL‑10, recombinant human IL‑10, and recombinant Epstein-Barr virus encoded viral IL‑10 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize IL‑10-mediated inhibition of LPS-induced IL-1 beta secretion in human peripheral blood lymphocytes (PBL). The Neutralization Dose (ND50) is typically 0.15-0.9 μg/mL in the presence of 2 ng/mL Recombinant Viral HCMV IL‑10. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Secretory expression of biologically active human Herpes virus interleukin-10 analogues in Escherichia coli via a modified Sec-dependent transporter construct. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant viral HCMV IL‑10 Ala26-Lys176 Accession # AAF63437 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Viral |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Interleukin-10 (IL-10) is a pleiotropic cytokine that plays a crucial role in regulating immune and inflammstory responses (1). It has closely related homologs in the genome of several viruses including the human cytomegalovirus (HCMV), a prevalent pathogen that can remain in humans as a latent infection (2). Viruses use multiple strategies to avoid detection and clearance by the host immune system. One of these strategies is to express virus-encoded host cytokine homologs, such as IL-10, to modulate the host immune system to the virus’ advantage. HCMV IL-10 is encoded from noncontiguous stretches in the viral genome and is transcribed early during infection. It shares 27% amino acid sequence identity with the endogenous human IL-10. Despite its low homology with the human protein, it can bind to the human IL-10 receptor complex and induces signal transduction. HCMV IL-10 has been shown to suppress cytokine production and inhibit proliferation of mitogen-stimulated human peripheral blood mononuclear cells. HCMV IL-10 can also down regulate the antigen-presenting and accessory cell functions of human monocytes. These immunosuppressive properties of HCMV IL-10 likely contribute to immune evasion during virus infection (3). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
IL-1 beta Secretion Inhibited by IL‑10 and Neutralization by Viral IL‑10 Antibody. Recombinant Viral HCMV IL‑10 (Catalog # 117-VL) inhibits LPS-indecued IL-1 beta secretion in human peripheral blood lymphocytes (PBL) in a dose-dependent manner (orange line). IL-1 beta secretion inhibited by Recombinant Viral HCMV IL‑10 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Viral HCMV IL‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF117). The ND50 is typically 0.15-0.9 μg/mL. |
