| 货号 | AF981-SP |
| 别名 | CRL2; CRL2cytokine receptor CRL2 precusor; CRLF2; CRLF2Y; CRLM-2; Cytokine receptor-like 2; cytokine receptor-like factor 2; ILXR; IL-XR; P2RY8/CRLF2 fusion; PCOR1; Thymic stromal lymphopoietin protein receptor; thymic stromal lymphopoietin receptor; thymic stromal-derived lymphopoietin receptor; TSLP receptor; TSLPR | 全称 | Thymic Stromal Lymphopoietin Receptor |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human TSLP R in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunocytochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize TSLP-induced proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑7 R alpha and TSLP R. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 0.3 ng/mL Recombinant Human TSLP. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 64109 (Human); 57914 (Mouse); 171499 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Thymic stromal lymphopoietin from trophoblasts induces dendritic cell-mediated regulatory TH2 bias in the decidua during early gestation in humans. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human TSLP R Val30-Phe232 Accession # Q9HC73 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | TSLP R, also named Delta (1) and CRLM-2 (2) (cytokine receptor-like module-2), was originally cloned as a novel type 1 cytokine receptor with similarity to the common gamma chain. It was subsequently identified to be a subunit of the cellular receptor for the IL-7-like cytokine TSLP and termed TSLP R (3). The human TSLP R cDNA encodes a 371 amino acid (aa) residue type 1 membrane protein with a 22 aa residue signal peptide, a 210 aa residue extracellular domain, a 20 aa residue transmembrane domain, and a 119 aa residue cytoplasmic domain (4, 5). The extracellular region contains two fibronectin type III-like domains and a WSXWS‑like motif. The cytoplasmic domain contains a membrane-proximal box 1 motif that is known to be important for association with JAKs (4). Human TSLP R displays 39% identity to mouse TSLP R and 24% identity to the common gamma receptor (4). An alternatively spliced mRNA variant encoding a soluble TSLP R has also been reported in mouse (2). TSLP R expression is ubiquitous in the immune and hematopoietic cells, but is up-regulated in Th2-skewed cells. Cells expressing TSLP R alone bind TSLP with low affinity. Co-expression of TSLP R and IL-7 R alpha is required for high-affinity TSLP binding and signal transduction (3-6). The TSLP R and IL-7 R alpha are coexpressed primarily on monocytes and dendritic cells and at lower levels in lymphoid cells. TSLP has been shown to induce the release of T cell‑attracting chemokines from monocytes and enhance the maturation of CD11c+ dendritic cells (5). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human TSLP R by Western Blot. Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TSLP R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF981) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TSLP R at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
TSLP R in Human Lymph Node. TSLP R was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-Human TSLP R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF981) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
Cell Proliferation Induced by TSLP and Neutralization by Human TSLP R Antibody. Recombinant Human TSLP (Catalog # 1398-TS) stimulates proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑7 R alpha and TSLP R in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human TSLP (0.3 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TSLP R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF981). The ND50 is typically 1‑5 µg/mL. |
