| 货号 | AF974-SP |
| 别名 | metalloproteinase inhibitor 4; TIMP metallopeptidase inhibitor 4; TIMP-4; tissue inhibitor of metalloproteinase 4; Tissue inhibitor of metalloproteinases 4 | 全称 | Tissue Inhibitors of Metalloproteinases 4 |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects human and mouse TIMP‑4 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human (rh) TIMP-1, rhTIMP-2, and rhTIMP-3 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7079 (Human); 110595 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Effect of bimatoprost, latanoprost, and unoprostone on matrix metalloproteinases and their inhibitors in human ciliary body smooth muscle cells. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human TIMP-4 Cys30-Pro224 Accession # Q99727 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Tissue inhibitors of metalloproteinases (TIMPs) are a family of secreted proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four known members of the family, TIMP-1, -2, -3, and -4. TIMP-4 is produced by a wide range of tissues, particularly brain, heart, ovary and skeletal muscle (1, 2). Limited studies have shown that TIMP-4 has a tumor suppressive effect against Wilm’s tumor, exhibits negative correlation with glioma maligancy and is found in breast carcinoma cells (3‑5). TIMP-4 inhibits MMP-mediated proteolysis by forming a non-covalent binary complex with the MMP active site through its N-terminal domain. In addition, it binds to the hemopexin-like domain of pro-MMP-2 through its C-terminal domain in a manner similar to TIMP-2 (6). However, unlike TIMP-2, TIMP-4 does not promote pro-MMP-2 activation by MT1-MMP (MMP-14) (7). Although TIMP-4 is a potent inhibitor of most MMPs, it is not an effective inhibitor of ADAMs, such as TACE (8, 9). Human TIMP-4 shares 89% sequence indentity with Mouse TIMP-4. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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