Human TIMP-1 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human TIMP-1 Affinity Purified Polyclonal Ab (25 UG)

货号： AF970-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF970-SP
别名	CLGI; Collagenase inhibitor; collagenase inhibitor); EPATIMP-1; EPO; erythroid potentiating activity; Erythroid-potentiating activity; Fibroblast collagenase inhibitor; FLJ90373; HCI; metalloproteinase inhibitor 1; TIMP metallopeptidase inhibitor 1; TIMPtissue inhibitor of metalloproteinase 1 (erythroid potentiating activity; Tissue inhibitor of metalloproteinases 1
全称	Tissue Inhibitors of Metalloproteinases 1
反应种属	Human
应用	Western Blot(1 µg/mL)
目标/特异性	Detects human TIMP-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse TIMP‑1 and recombinant rat TIMP-1 is observed and less than 1% cross-reactivity with recombinant human (rh) TIMP-2, rhTIMP-3, and rhTIMP‑4 is observed.
使用方法	Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize Recombinant Human TIMP-1 (0.1 µg/mL, Catalog # 970-TM) inhibition of Recombinant Human MMP‑2 (0.2 µg/mL, Catalog # 902-MP) cleavage of the fluorogenic peptide substrate Mca‑PLGL-Dpa-AR-NH₂ (5 µM, Catalog # ES001). The Neutralization Dose (ND₅₀) is typically 1 µg/mL.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7076 (Human); 21857 (Mouse); 116510 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Plasma levels of the MMP-9:TIMP-1 complex as prognostic biomarker in breast cancer: a retrospective study. Authors: Thorsen S, Christensen S, Wurtz S, Lundberg M, Nielsen B, Vinther L, Knowles M, Gee N, Fredriksson S, Moller S, Brunner N, Schrohl A, Stenvang J BMC Cancer, 2013;13(0):598. Species: Human Sample Type: Plasma Application: Proximity Ligation Assay Breast cancer cells induce cancer-associated fibroblasts to secrete hepatocyte growth factor to enhance breast tumorigenesis. Authors: Tyan SW, Kuo WH, Huang CK PLoS ONE, 2011;6(1):e15313. Species: Human Sample Type: Whole Cells Application: Neut Adipocytes promote ovarian cancer metastasis and provide energy for rapid tumor growth. Authors: Nieman KM, Kenny HA, Penicka CV, Ladanyi A, Buell-Gutbrod R, Zillhardt MR, Romero IL, Carey MS, Mills GB, Hotamisligil GS, Yamada SD, Peter ME, Gwin K, Lengyel E Nat. Med., 2011;17(11):1498-503. Species: Human Sample Type: In Vivo Application: In Vivo Homing Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation. Authors: Reikvam H, Hatfield KJ, Oyan AM, Kalland KH, Kittang AO, Bruserud O Eur. J. Haematol., 2010;84(3):239-51. Species: Human Sample Type: Whole Cells Application: Neut Fibroblast-conditioned media promote human sarcoma cell invasion. Authors: Bittner JG, Wilson M, Shah MB, Albo D, Feig BW, Wang TN Surgery, 2009;145(1):42-7. Species: Human Sample Type: Cell Lysates Application: WB Coregulation of vascular tube stabilization by endothelial cell TIMP-2 and pericyte TIMP-3. Authors: Saunders WB, Bohnsack BL, Faske JB, Anthis NJ, Bayless KJ, Hirschi KK, Davis GE J. Cell Biol., 2006;175(1):179-91. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human TIMP-1 Cys24-Ala207 Accession # Q6FGX5
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3 and TIMP-4. TIMP-1 is a glycoprotein with a molecular mass of 28 kDa produced by a wide range of cell types. TIMP-1 inhibits active MMP-mediated proteolysis by forming an N-terminal, non-covalent binary complex with the MMP active site. TIMP-1 also associates C-terminally with Pro-MMP-9 in a complex which may play a role in regulating activation. Independent of MMPs, TIMP-1 has been shown to have a role in tissue homeostasis.
运输条件	Blue Ice
存放说明	4℃
参考文献	Airway Inflammation and Remodeling Cancer Biomarkers ICC / IHC Images: TIMP-1 Inflammatory Mediators Metalloproteases and Regulators MMPs, TIMPs and Related Molecules OSM Induction of Chondrocyte MMP and TIMP-3 Gene Expression

参考图片

Detection of Human TIMP‑1 by Western Blot. Western blot shows lysates of human lung tissue and human prostate tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TIMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF970) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TIMP‑1 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Neutralization of TIMP‑1 Activity by Human TIMP‑1 Antibody. Recombinant Human MMP‑2 (0.2 µg/mL, Catalog # 902-MP) activity is measured in the presence of Recombinant Human TIMP-1 (0.1 µg/mL, Catalog # 970-TM) that has been preincubated with increasing concentrations of Goat Anti-Human TIMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF970). The ND₅₀ is typically 1 µg/mL.