| 货号 | AF962-SP |
| 别名 | B-cell stimulating factor 3; B-cell stimulatory factor 3; BSF-3; BSF3BSF-3; cardiotrophin-like cytokine factor 1; CISS2; CLCF1; CLCNNT-1; CRLF1 associated cytokine-like factor 1; neurotrophin-1/B-cell stimulating factor-3; NNT-1; NNT1B-cell-stimulating factor 3; Novel neurotrophin-1; NR6 | 全称 | Cardiotrophin-like Cytokine |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects human CLC in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CT-1 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 23529 (Human); 56708 (Mouse); 365395 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human CLC Leu28-Phe225 Accession # Q9UBD9 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Cardiotrophin-like cytokine (CLC) (also known as novel neurotrophin-1 (NNT-1) and B cell stimulating factor (BSF-3)) is a recently discovered 22-25 kDa member of the IL-6 family of cytokines (1, 2, 3). As such, it is expressed as a long type I cytokine with four alpha -helices in it structure (2). Human CLC is synthesized as a 225 amino acid precursor that contains a 27 aa signal sequence and a 198 aa mature region. It contains one potential N-linked glycosylation site that is apparently utilized, and two distinct binding sites for CNTFR alpha and CLF (4, 5). Although CLC has a signal sequence, it is not secreted unless noncovalently dimerized to either CLF or soluble CNTFR alpha (5, 6). Once dimerized, CLC signals through a tripartite receptor complex composed of gp130, LIFR beta and CNTFR alpha (membrane-bound) (5, 7). Within the IL-6 family, human CLC is most homologous to cardiotrophin-1, sharing approximately 29% amino acid sequence identity (8). Human to mouse, mature CLC is 96% aa identical. CLC is a trophic factor for motor neurons, a stimulator of ACTH release from corticotrophs, and an inducer of IgE synthesis and B cell proliferation (9, 10, 11). Cells known to express CLC include embryonic muscle, lung epithelium, and mesenchyme in various regions (12). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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