| 货号 | AF950-SP |
| 别名 | Constitutive NOS; EC 1.14.13.39; ECNOS; Endothelial NOS; endothelial; eNOScNOS; nitric oxide synthase 3 (endothelial cell); NOS type III; NOS3; NOSIII | 全称 | Endothelial Nitric Oxide Synthase |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Simple Western(10 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunoprecipitation(0.3 µg/106cells) |
| 目标/特异性 | Detects human eNOS in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Simple Western: 10 µg/mL Immunohistochemistry: 5-15 µg/mL Immunoprecipitation: 0.3 µg/106cells |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4846 (Human); 18127 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Shear stress-induced ATP-mediated endothelial constitutive nitric oxide synthase expression in human lymphatic endothelial cells. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human eNOS |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Endothelial NOS (eNOS), also known as nitric oxide synthase 3 (NOS3) or constitutive NOS (cNOS), is an enzyme encoded by the NOS3 gene. Endothelial NOS generates nitric oxide in blood vessels and is involved with regulating vascular tone by inhibiting smooth muscle contraction and platelet aggregation. A constitutive calcium dependent NOS provides a basal release of NO. eNOS is associated with plasma membranes surrounding cells and the membranes of the Golgi apparatus within cells. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human eNOS by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothlial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human eNOS Affinity-purified Polyclonal Antibody (Catalog # AF950) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for eNOS at approximately 130-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
Immunoprecipitation of Human eNOS. eNOS was immunoprecipitated from lysates (1 x 106 cells) of HUVEC human umbilical vein endothelial cells following incubation with 0.3 µg Goat Anti-Human eNOS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF950). Immunoprecipitated eNOS was detected by Western blot using 1 µg/mL Human eNOS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF950). View our recommended buffer recipes for immunoprecipitation. | |
Detection of Human eNOS by Simple WesternTM. Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, loaded at 0.2 mg/mL. A specific band was detected for eNOS at approximately 139 kDa (as indicated) using 10 µg/mL of Goat Anti-Human eNOS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF950) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system. |
