Human Desmoglein-2 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human Desmoglein-2 Affinity Purified Polyclonal Ab (25 UG)

货号： AF947-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF947-SP
别名	ARVC10; ARVD10; Cadherin family member 5; CDHF5; CDHF5MGC117034; CMD1BB; desmoglein 2; desmoglein-2; DSG2; HDGC; MGC117036; MGC117037
反应种属	Human
应用	Western Blot(0.25 µg/mL)
目标/特异性	Detects human Desmoglein-2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human Desmoglein-1 is observed.
使用方法	Western Blot: 0.25 µg/mL Simple Western: 5 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1829 (Human); 13511 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Chromatin structure of two genomic sites for targeted transgene integration in induced pluripotent stem cells and hematopoietic stem cells. Authors: van Rensburg, R, Beyer, I, Yao, X-Y, Wang, H, Denisenko, O, Li, Z-Y, Russell, D W, Miller, D G, Gregory, P, Holmes, M, Bomsztyk, K, Lieber, A Gene Ther, 2013;20(2):201-14. Species: Human Sample Type: whole cells Application: ICC A new human DSG2-transgenic mouse model for studying the tropism and pathology of human adenoviruses. Authors: Wang H, Beyer I, Persson J, Song H, Li Z, Richter M, Cao H, van Rensburg R, Yao X, Hudkins K, Yumul R, Zhang XB, Yu M, Fender P, Hemminki A, Lieber A J. Virol., 2012;86(11):6286-302. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Multimerization of adenovirus serotype 3 fiber knob domains is required for efficient binding of virus to desmoglein 2 and subsequent opening of epithelial junctions. Authors: Wang H, Li Z, Yumul R, Lara S, Hemminki A, Fender P, Lieber A J. Virol., 2011;85(13):6390-402. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human Desmoglein-2 Met1-Gly608 Accession # CAA81226
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Desmoglein-2 is one of three members of the desmoglein subfamily of calcium-dependent cadherin cell adhesion molecules. Together with desmocollins, another subfamily within the cadherin superfamily, the desmoglein isoforms form the adhesive components of desmosomes, the cell-cell adhesive structures that are found in epithelial cells. Human Desmoglein-2 is a type I transmembrane glycoprotein of 1117 amino acid (aa) residues with a 23 aa signal peptide and a 25 aa propeptide. It differs from other classic cadherins by having four instead of five cadherin repeat domains in its extracellular region, and a much larger cytoplasmic region containing five desmoglein repeat domains which share homology with the cadherin repeats. Instead of having the HAV adhesion motif found in type I cadherins, desmogleins have R/YAL as the adhesion motif on its amino-terminal cadherin repeat. The cytoplasmic tails of desmogleins interact with desmoplakins, plakoglobin and plakophilins. In turn, these proteins link the desmogleins with the intermediate filaments. Desmoglein-2 has been shown to be important in establishing cell-cell adhesion and function in epithelial cells. Desmoglein-2 was originally identified in colon carcinoma and colon, and was named HDGC (human desmoglein colon).
运输条件	Blue Ice
存放说明	4℃
参考文献	Nollet, R. et al. (2000) J. Mol. Biol. 299:551. Elias, P. et al. (2001) J. Cell Biol. 153:243. Arnemann, J. et al. (1992) Genomics 13:484.

参考图片

Detection of Human Desmoglein‑2 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line and A549 human lung carcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Desmoglein‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF947) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Desmoglein‑2 at approximately 90-160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.