Human VE-Cadherin Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human VE-Cadherin Affinity Purified Polyclonal Ab (25 UG)

货号： AF938-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF938-SP
别名	7B4 antigen; 7B4; cadherin 5, type 2 (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular epithelium); Cadherin-5; CD144 antigen; CD144; CDH5; endothelial-specific cadherin; FLJ17376; Vascular endothelial cadherin; VE-cadherin
全称	Vascular Endothelium Cadherin
反应种属	Human
应用	Western Blot(0.25 µg/mL) Flow Cytometry(2.5 µg/10⁶cells) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects human VE-Cadherin in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse VE‑Cadherin and recombinant human (rh) E-Cadherin is observed and less than 1% cross-reactivity with rhP-Cadherin is observed.
使用方法	Western Blot: 0.25 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1003 (Human); 12562 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. DeepCAGE transcriptomics identify HOXD10 as transcription factor regulating lymphatic endothelial responses to VEGF-C J Cell Sci, 2016;0(0):. Species: Human Sample Type: Cell Lysates Application: WB A novel serum-free monolayer culture for orderly hematopoietic differentiation of human pluripotent cells via mesodermal progenitors. Authors: Niwa A, Heike T, Umeda K PLoS ONE, 2011;6(7):e22261. Species: Human Sample Type: Whole Cells Application: ICC Signaling hierarchy regulating human endothelial cell development. Authors: Kelly MA, Hirschi KK Arterioscler. Thromb. Vasc. Biol., 2009;29(5):718-24. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human VE-Cadherin Asp48-Gln593 Accession # P33151
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Vascular endothelial (VE) - cadherin (VE-CAD), also called 7B4 and cadherin-5, is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium‑dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. VE-Cadherin has been shown to play important roles in vasculogenesis and angiogenesis. VE-cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Human VE-cadherin is a 784 amino acid (aa) residue protein with a 25 aa signal sequence and a 759 aa propeptide. The mature protein begins at amino acid 48 and has a 546 aa extracellular domain, a 27 aa transmembrane domain, and a 164 aa cytoplasmic domain. The human and mouse mature VE-CAD proteins share approximately 74% homology.
运输条件	Blue Ice
存放说明	4℃
参考文献	Shimoyama, Y. et al. (1989) J. Cell Biol. 109:1787. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123. Overduin, M. et al. (1995) Science 267:386. Takeichi, M. (1991) Science 251:1451. Nose, A. et al. (1987) EMBO J. 6:3655. Carmeliet, P. et al. (1999) Cell 98:147. Gory-Faure, S. et al. (1999) Development 126:2093.

参考图片

Detection of Human VE‑Cadherin by Western Blot. Western blot shows lysate of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human VE‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF938) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for VE‑Cadherin at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

VE‑Cadherin in HUVEC Cells. VE‑Cadherin was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Goat Anti-Human VE‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF938) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.