Human ADAM15 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human ADAM15 Affinity Purified Polyclonal Ab (25 UG)

货号： AF935-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF935-SP
别名	ADAM 15; ADAM metallopeptidase domain 15; disintegrin-like, and cysteine-rich protein 15; EC 3.4.24; MDC15; MDC-15; Metargidin
全称	A Disintegrin and Metalloprotease-like Domain 15
反应种属	Human
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunoprecipitation(25 µg/mL)
目标/特异性	Detects human ADAM15 Ectodomain in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse (rm) ADAM15 is observed, and less than 1% cross-reactivity with rmADAM9, rmADAM10, recombinant human (rh) ADAM8, rhADAM9, rhADAM10, rhADAM12, rhADAM17 (TACE), rhADAM19, rhADAM22, rhADAM23, rhADAM32, and rhADAM33 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunoprecipitation: 25 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	8751 (Human); 11490 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Alternative splicing of ADAM15 regulates its interactions with cellular SH3 proteins. Authors: Kleino I, Ortiz RM, Yritys M, Huovila AP, Saksela K J. Cell. Biochem., 2009;108(4):877-85. Species: Human Sample Type: Cell Lysates Application: WB ADAM15 upregulation and interaction with multiple binding partners in inflammatory bowel disease. Authors: Mosnier JF, Jarry A, Bou-Hanna C, Denis MG, Merlin D, Laboisse CL Lab. Invest., 2006;86(10):1064-73. Species: Human Sample Type: Tissue Homogenates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ADAM15 Ectodomain Leu18-Ser693 Accession # Q13444
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	ADAM15 belongs to a family of transmembrane proteins that containdisintegrin and metalloprotease domains. Members of this family havebeen implicated in cell adhesion via integrin binding and shedding ofcell surface molecules.
运输条件	Blue Ice
存放说明	4℃
参考文献	Airway Inflammation and Remodeling Cell to Cell Adhesion Disassembly During EMT Extracellular Matrix Remodeling and Cell Migration During EMT ICC / IHC Images: ADAM15 Metalloproteases and Regulators Mini-review: ADAMs

参考图片

ADAM15 in Human Prostate. ADAM15 was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human ADAM15 Ectodomain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF935) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.