Human MMP-7 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human MMP-7 Affinity Purified Polyclonal Ab (25 UG)

货号： AF907-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF907-SP
别名	EC 3.4.24; EC 3.4.24.23; Matrilysin; matrin; matrix metallopeptidase 7 (matrilysin, uterine); matrix metalloproteinase 7 (matrilysin, uterine); Matrix metalloproteinase-7; MMP-7; MPSL1; Pump-1 protease; PUMP1; PUMP-1; uterine matrilysin; Uterine metalloproteinase
全称	Matrix Metalloproteinase 7
反应种属	Human
应用	Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunoprecipitation(25 µg/mL)
目标/特异性	Detects human MMP-7 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse MMP-7 is observed, and less than 1% cross-reactivity with recombinant human (rh) MMP‑8 and rhMMP-12 is observed.
使用方法	Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunoprecipitation: 25 µg/mL Neutralization: Measured by its ability to neutralize Recombinant Human MMP‑7 (0.2 µg/mL, Catalog # 907-MP) cleavage of the fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH₂ (10 µM, Catalog # ES001). The Neutralization Dose (ND₅₀) is typically 2 µg/mL.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	4316 (Human); 17393 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Gelsolin induces colorectal tumor cell invasion via modulation of the urokinase-type plasminogen activator cascade. Authors: Zhuo, Jingli, Tan, Ee Hong, Yan, Benedict, Tochhawng, Lalchhan, Jayapal, Manikand, Koh, Shiuan, Tay, Hwee Kee, Maciver, Sutherla, Hooi, Shing Ch, Salto-Tellez, Manuel, Kumar, Alan Pre, Goh, Yaw Chon, Lim, Yaw Chyn, Yap, Celestia PLoS ONE, 2012;7(8):e43594. Species: Human Sample Type: Whole Cells Application: Neut Determination of matrilysin activity in gastrointestinal neoplasia. Authors: Hawinkels LJ, Verspaget HW, van den Berg M, Hanemaaijer R, Sier CF Eur. J. Clin. Invest., 2007;37(7):598-9. Species: Human Sample Type: Tissue Homogenates Application: ELISA Development
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human MMP‑7 Leu18-Lys267 Accession # P09237
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-7 (matrilysin) is expressed in epithelial cells of normal and diseased tissues, and is capable of digesting a large series of proteins of the extracellular matrix including collagen IV and X, gelatin, casein, laminin, aggrecan, entactin, elastin and versican. MMP-7 is implicated in the activation of other proteinases such as plasminogen, MMP-1, MMP-2, and MMP-9. In addition to its roles in connective tissue remodeling and cancer, MMP-7 also regulates intestinal alpha ‑defensin activation in innate host defense, releases tumor necrosis factor‑ alpha in a model of herniated disc resorption, and cleaves FasL to generate a soluble form in a model of prostate involution. Structurally, MMP-7 is the smallest of the MMPs and consists of two domains: a pro-domain that is cleaved upon activation and a catalytic domain containing the zinc-binding site.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cell to Cell Adhesion Disassembly During EMT Collagen Modifying Enzymes ICC / IHC Images: MMP-7 Inflammatory Mediators Metalloproteases and Regulators MMP Modulation of Cytokines MMP-12 in Smoking-Related Pulmonary Disease MMPs, TIMPs and Related Molecules New Tools: Active MMP Activity Assays OSM Induction of Chondrocyte MMP and TIMP-3 Gene Expression

参考图片

Neutralization of MMP‑7 Activity by Human MMP‑7 Antibody. The cleavage of
Mca‑PLGL‑Dpa‑AR‑NH₂ (10 μM, Catalog # ES001) by Recombinant Human MMP‑7 (0.2 µg/mL, Catalog # 907-MP) is measured after preincubation with increasing concentrations of Goat Anti-Human MMP‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF907). The ND₅₀ is typically 2 µg/mL.

Detection of Human MMP‑7 by Western Blot. Western blot shows lysates of Capan‑1 human pancreatic adenocarcinoma cell line and HT‑29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MMP‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF907) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MMP‑7 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

MMP‑7 in Human Pancreas. MMP‑7 was detected in immersion fixed paraffin-embedded sections of human pancreas array using Goat Anti-Human MMP‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF907) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.