Human IL-10 R beta Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human IL-10 R beta Affinity Purified Polyclonal Ab (25 UG)

货号： AF874-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF874-SP
别名	CDw210b antigen; CDw210b; CRF2-4IL-10R subunit 2; CRFB4; CRFB4human transmembrane receptor protein; cytokine receptor10CDW210B; D21S58; D21S66; IBD25; IL-10 receptor subunit beta; IL-10R2; IL-10R2IL-10R subunit beta; IL10RB; IL-10RB; interleukin 10 receptor, beta; Interleukin-10 receptor subunit 2; interleukin-10 receptor subunit beta; member 4
全称	Interleukin 10 Receptor beta
反应种属	Human
应用	Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects human IL-10 R beta in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) IL‑20 R alpha, rhIL-10 R, and rhIL-20 R beta is observed.
使用方法	Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Neutralization: Measured by its ability to neutralize IL‑10 R beta -mediated IL‑10 response in LPS-activated human peripheral blood mononuclear cells (PBMC). Ralph, P. et al. (1991) J. Immunology 148:808. The Neutralization Dose (ND₅₀) is typically 2-6 µg/mL in the presence of 0.25 ng/mL Recombinant Human IL‑10 and 0.25 ng/mL LPS.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3588 (Human); 16155 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Trisomy 21 consistently activates the interferon response Elife, 2016;5(0):. Species: Human Sample Type: Cell Lysates Application: WB HCV infection selectively impairs type I but not type III IFN signaling. Authors: Chandra P, Bao L, Song K, Aboulnasr F, Baker D, Shores N, Wimley W, Liu S, Hagedorn C, Fuchs S, Wu T, Balart L, Dash S Am J Pathol, 2014;184(1):214-29. Species: Human Sample Type: whole cells Application: Flow Despite IFN-lambda receptor expression, blood immune cells, but not keratinocytes or melanocytes, have an impaired response to type III interferons: implications for therapeutic applications of these cytokines. Authors: Witte K, Gruetz G, Volk HD, Looman AC, Asadullah K, Sterry W, Sabat R, Wolk K Genes Immun., 2009;10(8):702-14. Species: Human Sample Type: Cell Lysates Application: WB Biological activity of interleukins-28 and -29: comparison with type I interferons. Authors: Meager A, Visvalingam K, Dilger P, Bryan D, Wadhwa M Cytokine, 2005;31(2):109-18. Species: Human Sample Type: Whole Cells Application: Neut Cutting edge: IL-26 signals through a novel receptor complex composed of IL-20 receptor 1 and IL-10 receptor 2. Authors: Sheikh F, Baurin VV, Lewis-Antes A, Shah NK, Smirnov SV, Anantha S, Dickensheets H, Dumoutier L, Renauld JC, Zdanov A, Donnelly RP, Kotenko SV J. Immunol., 2004;172(4):2006-10. Species: Human Sample Type: Whole Cells Application: Neut Abnormal interleukin 10Ralpha expression contributes to the maintenance of elevated cyclooxygenase-2 in non-small cell lung cancer cells. Authors: Heuze-Vourc&amp;apos;h N Cancer Res., 2003;63(4):766-70. Species: Human Sample Type: Whole Cells Application: Flow IL-28, IL-29 and their class II cytokine receptor IL-28R. Authors: Sheppard P, Kindsvogel W, Xu W, Henderson K, Schlutsmeyer S, Whitmore TE, Kuestner R, Garrigues U, Birks C, Roraback J, Ostrander C, Dong D, Shin J, Presnell S, Fox B, Haldeman B, Cooper E, Taft D, Gilbert T, Grant FJ, Tackett M, Krivan W, McKnight G, Clegg C, Foster D, Klucher KM Nat. Immunol., 2002;4(1):63-8. Species: Human Sample Type: Whole Cells Application: Neut
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human IL-10 R beta Met20-Ser220 Accession # Q08334
内毒素水平	<0.20 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	IL-10, also known as IL-10 R2, mediates its biological activities via binding to a heteromeric receptor complex consisting of two distinct type II cytokine receptor subunits, the ligand binding IL-10 R alpha and the IL-10 R beta which does not bind IL-10 by itself but is required for signal transduction. The cDNA for human IL-10 R beta encodes a 325 amino acid (aa) residue type I transmembrane precursor protein with a 20 aa signal sequence, a 200 aa extracellular region, a 29 aa transmembrane segment, and a short 76 aa cytoplasmic domain. Within the extracellular region, there are two 100 aa subdomains that resemble the constant region of immunoglobulins. This structural motif is responsible for the alternative designation of IL-10 R beta as CRF2-4 (the 4^th member of the cytokine receptor family class II/2). Human and mouse IL-10 R beta share approximately 69% aa sequence identity. Binding of the non-covalent IL-10 dimer to two IL-10 R alpha chains recruits two IL‑10 R beta chains resulting in the activation and phosphorylation of the signaling cascade involving JAK1, TYK2, and STAT3. IL-10 R beta is expressed ubiquitously. IL‑10 R beta is also a component of the IL-22 receptor complex consisting of the IL-10 R beta chain and IL-22 R, another type II cytokine receptor family member.
运输条件	Blue Ice
存放说明	4℃
参考文献	Donnelly, R.P. et al. (2004) J. Leukoc. Biol. 76:314. Donnelly, R.P.et al. (1999) J. Interferon Cytokine Res. 19:563. Kotenko, S.V.et al. (2000) J. Biol. Chem. 276:2725. Liu, Y.et al. (1994) J. Immunol. 152:1821. Lutfalla, G.et al. (1993) Genomics 16:366. Kotenko, S.V.et al. (1997) EMBO J. 16:5894.

参考图片

IL‑10 Inhibition of IL‑1 beta secretion and Neutralization by Human IL‑10 R beta Antibody. Recombinant Human IL‑10 (Catalog # 217-IL) inhibits IL‑1 beta secretion in LPS-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line), as measured by the Human IL‑1 beta /IL‑1F2 Quantikine ELISA Kit (Catalog # DLB50).
IL‑1 beta secretion inhibited by Recombinant Human IL‑10 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑10 R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF874). The ND₅₀ is typically 2-6 µg/mL in the presence of LPS (0.25 ng/mL).