| 货号 | AF864-SP |
| 别名 | [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial; EC 2.7.11; EC 2.7.11.2; mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1; PDPK1; PKB kinase; Pyruvate dehydrogenase kinase isoform 1; pyruvate dehydrogenase kinase, isoenzyme 1; pyruvate dehydrogenase kinase, isozyme 1 | 全称 | Phosphoinositide Dependent Kinase-1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human PDK-1 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5170 (Human); 18607 (Mouse); 81745 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | KLH-coupled human PDK-1 synthetic peptide CKIIKLEYDFPEKFFPKARD Accession # AAD38505 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | PDK-1 (Phosphoinositide-dependent Kinase-1), also called PKB kinase and PDPK1, is a member of the AGC group of serine/threonine kinases. Following insulin, growth factor, or pervanadate stimulation, PDK-1 phosphorylates and activates kinases from the Akt, p70 S6K, and RSK families, and is therefore involved in the regulation of cell proliferation and apoptosis. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human PDK‑1 by Western Blot. Western blot shows lysates of WS‑1 human fetal skin fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human PDK‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF864) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PDK‑1 at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4. |
