Human Hrk Affinity Purified Polyclonal Ab (25 UG)
货号：
AF851-SP 基本售价：
1378.1 元 规格：
-
产品信息
概述| 货号 | AF851-SP |
| 别名 | activator of apoptosis harakiri; activator of apoptosis Hrk; BCL2-interacting protein; BH3-interacting domain-containing protein 3; BID3; death protein 5; DP5; HARAKIRI; harakiri, BCL2 interacting protein (contains only BH3 domain); harakiri, BCL2-interacting protein (contains only BH3 domain); Neuronal death protein DP5 |
全称 | Harakiri | | 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL)
|
| 目标/特异性 | Detects human Hrk in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL
|
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 8739 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | KLH-coupled human Hrk synthetic peptide
RTMWRRRARSR RAPAPGAALPC |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Activator of apoptosis Hrk regulates apoptosis through interaction with death-repressor proteins Bcl-2 and Bcl-X(L). The HRK protein lacks significant homology to other BCL2 family members except for an 8-amino acid region that was similar to the BCL2 homology domain-3 (BH3) motif of BIK. HRK interacts with BCL2 and BCLXL via the BH3 domain, but not with the death-promoting BCL2-related proteins BAX, BAK, or BCLXS. HRK localizes to membranes of intracellular organelles in a pattern similar to that previously reported for BCL2 and BCLXL. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Bcl-2 Proteins and Regulators
|
参考图片Detection of Human Hrk by Western Blot. Western blot shows lysates of E. coli expressing recombinant human Hrk-green fluorescent protein fusion. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human Hrk Antigen Affinity-purified Polyclonal Antibody (Catalog # AF851) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Hrk was detected at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4. |
