| 货号 | AF8516-SP |
| 别名 | EC 2.7.1.36; LH receptor mRNA-binding protein; LRBP; mevalonate kinase (mevalonic aciduria); mevalonate kinase 1; mevalonate kinase; Mevalonic Aciduria; MKFLJ96772; MVLK; POROK3 | 全称 | Mevalonate Kinase |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Simple Western(1 µg/mL) Immunoprecipitation(4 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human MVK in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.1 µg/mL Simple Western: 1 µg/mL Immunoprecipitation: 4 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4598 (Human); 17855 (Mouse); 81727 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human MVK Met1-Lys396 Accession # Q03426 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | MVK (Mevalonate kinase) is a 42 kDa cytoplasmic protein that belongs to the GHMP kinase family, Mevalonate kinase subfamily of proteins. Mevalonate kinase catalyzes the ATP-dependent phosphorylation of mevalonic acid to form mevalonate 5-phosphate. Defects in mevalonate kinase can cause mevalonic aciduria (MEVA). It is an accumulation of mevalonic acid which causes a variety of symptoms such as psychomotor retardation, dysmorphic features, cataracts, hepatosplenomegaly, lymphadenopathy, anemia, hypotonia, myopathy and ataxia.Over aa 1-396, human MVK shares 81% aa identity with mouse MVK. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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MVK in A431 Human Cell Line. MVK was detected in immersion fixed A431 human epithelial carcinoma cell line using Rabbit Anti-Human MVK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8516) at 2 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
Detection of Human MVK by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, A431 human epithelial carcinoma cell line, and HEK001 human epidermal keratinocyte cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human MVK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8516) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for MVK at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Immunoprecipitation of Human MVK. MVK was immunoprecipitated from 400 µg of HepG2 human hepatocellular carcinoma cell line and A431 human epithelial carcinoma cell line lysates using 4 µg of Rabbit Anti-Human MVK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8516) coated on 4 wells of a 96 well plate (Corning Costar EIA/RIA). HepG2 lysates, A431 lysates, Rabbit IgG control buffer, or recombinant human MVK were added to the wells and incubated for 2 hours at room temperature. Immunoprecipitated MVK was detected by Western blot under reducing conditions using 0.1 µg/mL Rabbit Anti-Human MVK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8516) and Immunoblot Buffer Group 1. View our recommended buffer recipes for Immunoprecipitation. | |
Detection of Human MVK by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and HEK001 human epidermal keratinocyte cell line, loaded at 0.2 mg/mL. A specific band was detected for MVK at approximately 43 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human MVK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8516). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
