| 货号 | AF8515-SP |
| 别名 | Kazal type 5; LETKI; NETS; serine peptidase inhibitor, Kazal type 5; SPINK5; VAKTI | 全称 | Serine Peptidase Inhibitor, Kazal Type 5 |
| 反应种属 | Human |
| 应用 | Western Blot(0.5 µg/mL) |
| 目标/特异性 | Detects human SPINK5 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 11005 (Human); 72432 (Mouse); 361319 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Human embyronic kidney cell line HEK293-derived recombinant human SPINK5 Glu626-Glu1064 Accession # Q9NQ38 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | LEKTI (Lympho-epithelial Kazal-type-related inhibitor; also SPINK5, Serine Protease Inhibitor Kazal-type 5) is a 150 kDa precursor protein that is cleaved into the following 2 chains: Hemofiltrate peptide HF6478 and Hemofiltrate peptide HF7665. LEKTI is a multidomain serine protease inhibitor that contains 15 Kazal-like inhibitory domains. It may play a role in skin and hair morphogenesis and anti-inflammatory and/or antimicrobial protection of mucous epithelia. Mutations may result in Netherton syndrome, a disorder characterized by ichthyosis, defective cornification, and atopy. It contains fifteen Kazal-like domains. Mature human SPINK5 shares 60% aa sequence identity with mouse SPINK5. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human SPINK5 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and COLO 205 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human SPINK5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8515) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for SPINK5 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
